Analysis of gene expression in the developing mouse retina

Elva D Diaz, Yee Hwa Yang, Todd Ferreira, Kenneth C. Loh, Yasushi Okazaki, Yoshihide Hayashizaki, Marc Tessier-Lavigne, Terence P. Speed, John Ngai

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Abstract

In the visual system, differential gene expression underlies development of the anterior-posterior and dorsal-ventral axes. Here we present the results of a microarray screen to identify genes differentially expressed in the developing retina. We assayed gene expression in nasal (anterior), temporal (posterior), dorsal, and ventral embryonic mouse retina. We used a statistical method to estimate gene expression between different retina regions. Genes were clustered according to their expression pattern and were ranked within each cluster. We identified groups of genes expressed in gradients or with restricted patterns of expression as verified by in situ hybridization. A common theme for the identified genes is the differential expression in the dorsal-ventral axis. By analyzing gene expression patterns, we provide insight into the molecular organization of the developing retina.

Original languageEnglish (US)
Pages (from-to)5491-5496
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number9
DOIs
StatePublished - Apr 29 2003

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ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Diaz, E. D., Yang, Y. H., Ferreira, T., Loh, K. C., Okazaki, Y., Hayashizaki, Y., Tessier-Lavigne, M., Speed, T. P., & Ngai, J. (2003). Analysis of gene expression in the developing mouse retina. Proceedings of the National Academy of Sciences of the United States of America, 100(9), 5491-5496. https://doi.org/10.1073/pnas.0831080100