Abstract
Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 193-204 |
| Number of pages | 12 |
| Journal | Journal of Autoimmunity |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 1996 |
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Keywords
- Crohn's disease
- Inflammatory bower disease
- Mucosal T cells
- T cell receptors
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease. / Prindiville, Thomas P; Cantrell, Mary C.; Matsumoto, Takayuki; Brown, William R.; Ansari, Aftab A.; Kotzin, Brian L.; Gershwin, M. Eric.
In: Journal of Autoimmunity, Vol. 9, No. 2, 04.1996, p. 193-204.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease
AU - Prindiville, Thomas P
AU - Cantrell, Mary C.
AU - Matsumoto, Takayuki
AU - Brown, William R.
AU - Ansari, Aftab A.
AU - Kotzin, Brian L.
AU - Gershwin, M. Eric
PY - 1996/4
Y1 - 1996/4
N2 - Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.
AB - Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.
KW - Crohn's disease
KW - Inflammatory bower disease
KW - Mucosal T cells
KW - T cell receptors
UR - http://www.scopus.com/inward/record.url?scp=0029956261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029956261&partnerID=8YFLogxK
U2 - 10.1006/jaut.1996.0023
DO - 10.1006/jaut.1996.0023
M3 - Article
C2 - 8738963
AN - SCOPUS:0029956261
VL - 9
SP - 193
EP - 204
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
SN - 0896-8411
IS - 2
ER -