Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease

Thomas P Prindiville, Mary C. Cantrell, Takayuki Matsumoto, William R. Brown, Aftab A. Ansari, Brian L. Kotzin, M. Eric Gershwin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.

Original languageEnglish (US)
Pages (from-to)193-204
Number of pages12
JournalJournal of Autoimmunity
Volume9
Issue number2
DOIs
StatePublished - Apr 1996

Keywords

  • Crohn's disease
  • Inflammatory bower disease
  • Mucosal T cells
  • T cell receptors

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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