Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease

Thomas P Prindiville, Mary C. Cantrell, Takayuki Matsumoto, William R. Brown, Aftab A. Ansari, Brian L. Kotzin, M. Eric Gershwin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.

Original languageEnglish (US)
Pages (from-to)193-204
Number of pages12
JournalJournal of Autoimmunity
Volume9
Issue number2
DOIs
StatePublished - Apr 1996

Fingerprint

T-Cell Antigen Receptor Specificity
Crohn Disease
Clone Cells
T-Lymphocytes
Cell Line
T-Cell Receptor Genes
Mucins
Antigens
Interleukin-2
Colon
Tetanus Toxoid
Microvilli
Thymidine
Mucous Membrane
Phenotype
Population

Keywords

  • Crohn's disease
  • Inflammatory bower disease
  • Mucosal T cells
  • T cell receptors

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease. / Prindiville, Thomas P; Cantrell, Mary C.; Matsumoto, Takayuki; Brown, William R.; Ansari, Aftab A.; Kotzin, Brian L.; Gershwin, M. Eric.

In: Journal of Autoimmunity, Vol. 9, No. 2, 04.1996, p. 193-204.

Research output: Contribution to journalArticle

Prindiville, Thomas P ; Cantrell, Mary C. ; Matsumoto, Takayuki ; Brown, William R. ; Ansari, Aftab A. ; Kotzin, Brian L. ; Gershwin, M. Eric. / Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease. In: Journal of Autoimmunity. 1996 ; Vol. 9, No. 2. pp. 193-204.
@article{f9d3c1004be54d63bec262f33e036965,
title = "Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease",
abstract = "Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5{\%} or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.",
keywords = "Crohn's disease, Inflammatory bower disease, Mucosal T cells, T cell receptors",
author = "Prindiville, {Thomas P} and Cantrell, {Mary C.} and Takayuki Matsumoto and Brown, {William R.} and Ansari, {Aftab A.} and Kotzin, {Brian L.} and Gershwin, {M. Eric}",
year = "1996",
month = "4",
doi = "10.1006/jaut.1996.0023",
language = "English (US)",
volume = "9",
pages = "193--204",
journal = "Journal of Autoimmunity",
issn = "0896-8411",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Analysis of function, specificity and T cell receptor expression of cloned mucosal T cell lines in Crohn's disease

AU - Prindiville, Thomas P

AU - Cantrell, Mary C.

AU - Matsumoto, Takayuki

AU - Brown, William R.

AU - Ansari, Aftab A.

AU - Kotzin, Brian L.

AU - Gershwin, M. Eric

PY - 1996/4

Y1 - 1996/4

N2 - Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.

AB - Monoclonal populations of mucosal T cells were established from the earliest visible lesions in eight patients with well defined Crohn's disease. The FAGS phenotype of all the mucosal derived clones to date are TCR α/β+, CD3+, CD4+, and CD45RO+ memory cells. TCR variable region Beta chain analysis revealed predominantly Vβ families 1, 2, 5.1, 5.2, 6, 7 and 8, with Vβ family analysis supporting antigen expansion in the diseased mucosa. Putative autoreactivity was evaluated by stimulating individual clones with a battery of antigens and determining proliferation and IL-2 production by thymidine incorporation at 72 h. Antigens tested included crude Crohn's diseased (CD) colon and small bowel homogenates, CD brush border preparations, crude CD colon and small bowel mucin, and purified CD small bowel mucin. Controls included clone, APC, tetanus toxoid and either PHA or Staphylococcus enterotoxin B. A total of 200 clones were studied with 29.5% or 59 clones demonstrating proliferation and/or IL-2 production. T cell receptor Vβ gene usage evaluated in a small number of reactive clones correlated with the expanded patient families. Seven of the fifteen represented families revealed diverse T cell receptor gene use and no disease overlap.

KW - Crohn's disease

KW - Inflammatory bower disease

KW - Mucosal T cells

KW - T cell receptors

UR - http://www.scopus.com/inward/record.url?scp=0029956261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029956261&partnerID=8YFLogxK

U2 - 10.1006/jaut.1996.0023

DO - 10.1006/jaut.1996.0023

M3 - Article

VL - 9

SP - 193

EP - 204

JO - Journal of Autoimmunity

JF - Journal of Autoimmunity

SN - 0896-8411

IS - 2

ER -