Analysis of altered capillary pressure and permeability after thermal injury

R. M. Pitt, J. C. Parker, Gregory Jurkovich, A. E. Taylor, P. W. Curreri

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In order to investigate the effects of thermal injury on microvascular hemodynamics and permeability, hindpaw arterial (PA), venous (PV), and capillary (PC) pressures, blood (QB) and lymph (QL) flows, and lymph (CL) and plasma (CP) total protein concentrations were measured before and for 3 hr after a 10-sec 100°C scald burn in 11 dogs. Prior to injury in eight experiments (Group I-permeability analysis) venous pressure was elevated by outflow restriction until the minimal CL CP was obtained. In three experiments (Group II-hemodynamic analysis) outflow was not restricted. Lymph and plasma protein fractions ranging in size from 37 to 120 Å were measured using gradient gel electrophoresis and capillary equivalent pore sizes were calculated. In the early postburn period, PC increased from 24 ± 2 (mean ± SE)to 47 ± 5 mm Hg (P < 0.05) and precapillary resistance (RA) decreased from 6.6 ± 0.2 to 2.5 ± 0.2 mm Hg/ml/min/100 g (P < 0.05) while postcapillary resistance (RV) remained unchanged. Pre- to postcapillary resistance ( RA RV) fell by 74%. The reflection coefficient for total proteins (calculated as σ = 1 - CL CP) decreased from 0.87 ± 0.01 to 0.45 ± 0.02 (P < 0.01). Permeability of the postburn capillary endothelium was described by using two populations of equivalent pores. Preburn pore radii were 50 and 300 Å with 13% of the capillary filtrate passing through the large pores. Pore radii increased after injury to 70 and 400 Å with 49% of the filtrate passing through the large pores. The postburn total tissue filtration coefficient (Kf) increased to 2.4 times the control. Over the first 3 hr postburn, 53% of the increase in capillary filtration was attributable to increased capillary pressure and 47% to increased permeability. We conclude that the early rapid edema formation following thermal injury is the result of marked increases in both capillary filtration pressure and filtration through large nonsieving pores.

Original languageEnglish (US)
Pages (from-to)693-702
Number of pages10
JournalJournal of Surgical Research
Volume42
Issue number6
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Capillary Permeability
Hot Temperature
Pressure
Wounds and Injuries
Lymph
Permeability
Venous Pressure
Hemodynamics
Vascular Endothelium
Capillary Electrophoresis
Blood Proteins
Edema
Gels
Dogs
Population
Proteins

ASJC Scopus subject areas

  • Surgery

Cite this

Analysis of altered capillary pressure and permeability after thermal injury. / Pitt, R. M.; Parker, J. C.; Jurkovich, Gregory; Taylor, A. E.; Curreri, P. W.

In: Journal of Surgical Research, Vol. 42, No. 6, 01.01.1987, p. 693-702.

Research output: Contribution to journalArticle

Pitt, R. M. ; Parker, J. C. ; Jurkovich, Gregory ; Taylor, A. E. ; Curreri, P. W. / Analysis of altered capillary pressure and permeability after thermal injury. In: Journal of Surgical Research. 1987 ; Vol. 42, No. 6. pp. 693-702.
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abstract = "In order to investigate the effects of thermal injury on microvascular hemodynamics and permeability, hindpaw arterial (PA), venous (PV), and capillary (PC) pressures, blood (QB) and lymph (QL) flows, and lymph (CL) and plasma (CP) total protein concentrations were measured before and for 3 hr after a 10-sec 100°C scald burn in 11 dogs. Prior to injury in eight experiments (Group I-permeability analysis) venous pressure was elevated by outflow restriction until the minimal CL CP was obtained. In three experiments (Group II-hemodynamic analysis) outflow was not restricted. Lymph and plasma protein fractions ranging in size from 37 to 120 {\AA} were measured using gradient gel electrophoresis and capillary equivalent pore sizes were calculated. In the early postburn period, PC increased from 24 ± 2 (mean ± SE)to 47 ± 5 mm Hg (P < 0.05) and precapillary resistance (RA) decreased from 6.6 ± 0.2 to 2.5 ± 0.2 mm Hg/ml/min/100 g (P < 0.05) while postcapillary resistance (RV) remained unchanged. Pre- to postcapillary resistance ( RA RV) fell by 74{\%}. The reflection coefficient for total proteins (calculated as σ = 1 - CL CP) decreased from 0.87 ± 0.01 to 0.45 ± 0.02 (P < 0.01). Permeability of the postburn capillary endothelium was described by using two populations of equivalent pores. Preburn pore radii were 50 and 300 {\AA} with 13{\%} of the capillary filtrate passing through the large pores. Pore radii increased after injury to 70 and 400 {\AA} with 49{\%} of the filtrate passing through the large pores. The postburn total tissue filtration coefficient (Kf) increased to 2.4 times the control. Over the first 3 hr postburn, 53{\%} of the increase in capillary filtration was attributable to increased capillary pressure and 47{\%} to increased permeability. We conclude that the early rapid edema formation following thermal injury is the result of marked increases in both capillary filtration pressure and filtration through large nonsieving pores.",
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