Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces

Tennessee J. Yoder, Mark A. McElwain, Susan E. Francis, Joy Bagley, Eric G.D. Muller, Brian Pak, Eileen T. O'Toole, Mark Winey, Trisha N. Davis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The spindle pole body (SPB) is the microtubule organizing center in Saccharomyces cerevisiae. An essential task of the SPB is to ensure assembly of the bipolar spindle, which requires a proper balancing of forces on the microtubules and chromosomes. The SPB component Spc110p connects the ends of the spindle microtubules to the core of the SPB. We previously reported the isolation of a mutant allele spc110-226 that causes broken spindles and SPB disintegration 30 min after spindle formation. By live cell imaging of mutant cells with green fluorescent protein (GFP)-Tub1p or Spc97p-GFP, we show that spc110-226 mutant cells have early defects in spindle assembly. Short spindles form but do not advance to the 1.5-μm stage and frequently collapse. Kinetochores are not arranged properly in the mutant cells. In 70% of the cells, no stable biorientation occurs and all kinetochores are associated with only one SPB. Examination of the SPB remnants by electron microscopy tomography and fluorescence microscopy revealed that the Spc110-226p/calmodulin complex is stripped off of the central plaque of the SPB and coalesces to from a nucleating structure in the nucleoplasm. The central plaque components Spc42p and Spc29p remain behind in the nuclear envelope. The delamination is likely due to a perturbed interaction between Spc42p and Spc110-226p as detected by fluorescence resonance energy transfer analysis. We suggest that the force exerted on the SPB by biorientation of the chromosomes pulls the Spc110-226p out of the SPB; removal of force exerted by coherence of the sister chromatids reduced fragmentation fourfold. Removal of the forces exerted by the cytoplasmic microtubules had no effect on fragmentation. Our results provide insights into the relative contributions of the kinetochore and cytoplasmic microtubules to the forces involved in formation of a bipolar spindle.

Original languageEnglish (US)
Pages (from-to)141-152
Number of pages12
JournalMolecular Biology of the Cell
Volume16
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

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Spindle Pole Bodies
Kinetochores
Microtubules
Green Fluorescent Proteins
Chromosomes
Electron Microscope Tomography
Microtubule-Organizing Center
Fluorescence Resonance Energy Transfer
Chromatids
Nuclear Envelope
Calmodulin
Fluorescence Microscopy
Saccharomyces cerevisiae
Electron Microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Yoder, T. J., McElwain, M. A., Francis, S. E., Bagley, J., Muller, E. G. D., Pak, B., ... Davis, T. N. (2005). Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces. Molecular Biology of the Cell, 16(1), 141-152. https://doi.org/10.1091/mbc.E04-08-0703

Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces. / Yoder, Tennessee J.; McElwain, Mark A.; Francis, Susan E.; Bagley, Joy; Muller, Eric G.D.; Pak, Brian; O'Toole, Eileen T.; Winey, Mark; Davis, Trisha N.

In: Molecular Biology of the Cell, Vol. 16, No. 1, 01.01.2005, p. 141-152.

Research output: Contribution to journalArticle

Yoder, TJ, McElwain, MA, Francis, SE, Bagley, J, Muller, EGD, Pak, B, O'Toole, ET, Winey, M & Davis, TN 2005, 'Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces', Molecular Biology of the Cell, vol. 16, no. 1, pp. 141-152. https://doi.org/10.1091/mbc.E04-08-0703
Yoder, Tennessee J. ; McElwain, Mark A. ; Francis, Susan E. ; Bagley, Joy ; Muller, Eric G.D. ; Pak, Brian ; O'Toole, Eileen T. ; Winey, Mark ; Davis, Trisha N. / Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 1. pp. 141-152.
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