Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers

R. V. Kesseli, I. Paran, Richard W Michelmore

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

A detailed genetic map has been constructed from the F2 population of a single intraspecific cross of Lactuca sativa (n = 9). It comprises 319 loci, including 152 restriction fragment length polymorphism (RFLP), 130 random amplified polymorphic DNA (RAPD), 7 isozyme, 19 disease resistance, and 11 morphological markers. Thirteen major, four minor linkage groups and several unlinked markers are identified for this genome which is estimated to be approximately 1950 cM. RFLP and RAPD markers show similar distributions throughout the genome and identified similar levels of polymorphism. RAPD loci were much quicker to identify but more difficult to order. Procedures for generating accurate genetic maps and their limitations are described.

Original languageEnglish (US)
Pages (from-to)1435-1446
Number of pages12
JournalGenetics
Volume136
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

Lettuce
Genetic Linkage
Genetic Markers
Restriction Fragment Length Polymorphisms
Genome
Disease Resistance
DNA
Isoenzymes
Population

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Kesseli, R. V., Paran, I., & Michelmore, R. W. (1994). Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. Genetics, 136(4), 1435-1446.

Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. / Kesseli, R. V.; Paran, I.; Michelmore, Richard W.

In: Genetics, Vol. 136, No. 4, 1994, p. 1435-1446.

Research output: Contribution to journalArticle

Kesseli, RV, Paran, I & Michelmore, RW 1994, 'Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers', Genetics, vol. 136, no. 4, pp. 1435-1446.
Kesseli, R. V. ; Paran, I. ; Michelmore, Richard W. / Analysis of a detailed genetic linkage map of Lactuca sativa (lettuce) constructed from RFLP and RAPD markers. In: Genetics. 1994 ; Vol. 136, No. 4. pp. 1435-1446.
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