Analysis of a commercial dimethyl-sulfoxide-stabilized frozen canine platelet concentrate by turbidimetric aggregometry

Julien Guillaumin, Karl Jandrey, Jeffrey W. Norris, Fern Tablin

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Objectives- To assess platelet function of a commercial dimethyl-sulfoxide (DMSO)-stabilized frozen platelet concentrate (PC) using turbidimetric aggregometry.Design- In vitro analysis.Setting- Research laboratory in a school of veterinary medicine.Animals- Five units of frozen PC in 6% DMSO were studied. Fresh platelet-rich plasma (PRP), with and without 6% DMSO, from 6 healthy dogs were used as controls.Interventions- Turbidimetric platelet aggregation was measured after initiation of platelet aggregation by addition of adenosine diphosphate (ADP), collagen, or thrombin at concentrations of 30 μM, 20 μg/mL, and 0.5 U/mL, respectively. Measures were performed at thaw and repeated 2 hours after thaw for the frozen PC.Measurements and Main Results- Compared with PRP, the frozen PC showed decreased aggregation in response to thrombin (amplitude of 84% versus 25%, P=0.01), and collagen (amplitude of 13% versus 3%, P=0.05) but not ADP (6.5% versus 18%, P=0.2). Compared with frozen PC at thaw, the frozen PC at 2 hours after thaw showed decreased aggregation in response to thrombin, collagen, and ADP (P<0.05). There was no difference in aggregation between PRP in 6% DMSO and frozen PC.Conclusions- These in vitro data suggest there is a decrease in platelet response to agonists associated with the freeze-thaw process in the commercially available 6% DMSO canine frozen PC.

Original languageEnglish (US)
Pages (from-to)571-577
Number of pages7
JournalJournal of Veterinary Emergency and Critical Care
Volume20
Issue number6
DOIs
StatePublished - Dec 2010

Keywords

  • Coagulation
  • Critical care
  • Fluid therapy
  • Hemostasis
  • Platelet function
  • Transfusion medicine

ASJC Scopus subject areas

  • veterinary(all)

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