Analysis of 8 sarcomeric candidate genes for feline hypertrophic cardiomyopathy mutations in cats with hypertrophic cardiomyopathy

K. M. Meurs, M. M. Norgard, M. Kuan, J. Haggstrom, Mark D Kittleson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Hypertrophic cardiomyopathy (HCM) is the most common heart disease in cats. Causative mutations have been identified in the Maine Coon (MC) and Ragdoll breed in the cardiac myosin binding protein C gene (MYBPC3). HCM is thought to be inherited in other breeds. Hypothesis: That a causative mutation for HCM in the British Shorthair (BSH), Norwegian Forest (NWF), Siberian, Sphynx, or MC cats would be identified in the exonic and splice site regions of 1 of 8 genes associated with human familial HCM. Animals: Three affected BSH, NWF, Siberians, Sphynx, 2 MC (without the known MC mutation), and 2 Domestic Shorthair cats (controls) were studied. Methods: Prospective, observational study. Exonic and splice site regions of the genes encoding the proteins cardiac troponin I, troponin T, MYBPC3, cardiac essential myosin light chain, cardiac regulatory myosin light chain, α tropomyosin, actin, and β-myosin heavy chain were sequenced. Sequences were compared for nucleotide changes between affected cats, the published DNA sequences, and control cats. Changes were considered to be causative for HCM if they involved a conserved amino acid and changed the amino acid to a different polarity, acid-base status, or structure. Results: A causative mutation for HCM was not identified, although several single nucleotide polymorphisms were detected. Conclusions and Clinical Importance: Mutations within these cardiac genes do not appear to be the only cause of HCM in these breeds. Evaluation of additional cardiac genes is warranted to identify additional molecular causes of this feline cardiac disease.

Original languageEnglish (US)
Pages (from-to)840-843
Number of pages4
JournalJournal of Veterinary Internal Medicine
Volume23
Issue number4
DOIs
StatePublished - Jul 2009

Fingerprint

Hypertrophic Cardiomyopathy
cardiomyopathy
Felidae
Cats
cats
Cardiac Myosins
mutation
Mutation
Genes
Myosin Light Chains
genes
myosin light chains
heart diseases
breeds
Heart Diseases
Familial Hypertrophic Cardiomyopathy
Cat Diseases
Amino Acids
Tropomyosin
Troponin T

Keywords

  • Cardiovascular
  • Genetics
  • Molecular biology
  • Nucleic acid sequencing

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Analysis of 8 sarcomeric candidate genes for feline hypertrophic cardiomyopathy mutations in cats with hypertrophic cardiomyopathy. / Meurs, K. M.; Norgard, M. M.; Kuan, M.; Haggstrom, J.; Kittleson, Mark D.

In: Journal of Veterinary Internal Medicine, Vol. 23, No. 4, 07.2009, p. 840-843.

Research output: Contribution to journalArticle

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