Analysis and quantitation of fructooligosaccharides using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry

Richard R. Seipert, Mariana Barboza Gardner, Milady R. Niñonuevo, Riccardo G. LoCascio, David A. Mills, Samara L. Freeman, J. Bruce German, Carlito B Lebrilla

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Inulin is a class of fructooligosaccharide (FOS) derived from plants, which is often used as a natural food ingredient. Inulin is currently used as an additive in baked goods, dairy products, infant formula, and dietary supplements as a result of its purported health-promoting properties. The growth of health-promoting lactobacilli and bifidobacteria is supported by FOS, giving it the classification of a prebiotic; however, its ability to selectivity stimulate only beneficial bacteria has not been demonstrated. In order to better understand the role of inulin and FOS as prebiotics, matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry has been used for qualitative and quantitative analysis on bacterial growth. A method using an internal standard has been developed to quantify the consumption of FOS by Bifidobacterium longum bv. infantis using a calibration curve. Due to the differential consumption of FOS, the calibration curve was modified to include intensity components for each polymer unit in order to achieve more accurate quantitation. The method described was designed to be more rapid, precise, and robust for quantitative analysis when compared to existing methods.

Original languageEnglish (US)
Pages (from-to)159-165
Number of pages7
JournalAnalytical Chemistry
Volume80
Issue number1
DOIs
StatePublished - Jan 1 2008

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Cyclotron resonance
Ionization
Mass spectrometry
Desorption
Fourier transforms
Inulin
Ions
Lasers
Prebiotics
Dietary supplements
Health
Calibration
Dairy products
Chemical analysis
Bacteria
Polymers
fructooligosaccharide

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Analysis and quantitation of fructooligosaccharides using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry. / Seipert, Richard R.; Barboza Gardner, Mariana; Niñonuevo, Milady R.; LoCascio, Riccardo G.; Mills, David A.; Freeman, Samara L.; German, J. Bruce; Lebrilla, Carlito B.

In: Analytical Chemistry, Vol. 80, No. 1, 01.01.2008, p. 159-165.

Research output: Contribution to journalArticle

Seipert, Richard R. ; Barboza Gardner, Mariana ; Niñonuevo, Milady R. ; LoCascio, Riccardo G. ; Mills, David A. ; Freeman, Samara L. ; German, J. Bruce ; Lebrilla, Carlito B. / Analysis and quantitation of fructooligosaccharides using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry. In: Analytical Chemistry. 2008 ; Vol. 80, No. 1. pp. 159-165.
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