Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis

Yanlei Liu, Kan Lam, Yajarayma J. Tang, Paul H. Gumerlock, Dong Ki Lee, Myung Hwa Kim, Sum P. Lee, Joseph Silva, Joseph Leung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83%, 49/59). The two most common groups were detected in 19 (41%) and 17 (37%) of the 46 samples using SSPC analysis, and 25/59 (42%) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalChinese Medical Journal
Volume113
Issue number9
StatePublished - Sep 2000

Fingerprint

Bacteroides fragilis
Anaerobic Bacteria
Clostridium
rRNA Genes
Polymerase Chain Reaction
Glutamate-Ammonia Ligase
Molecular Biology
DNA
Genes

Keywords

  • Anaerobic bacteria
  • Intrahepatic stone
  • PCR
  • Single-strand confromational polymorphism

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Liu, Y., Lam, K., Tang, Y. J., Gumerlock, P. H., Lee, D. K., Kim, M. H., ... Leung, J. (2000). Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis. Chinese Medical Journal, 113(9), 858-861.

Anaerobic bacteria and intrahepatic stones : Detections of Clostridium sp. and Bacteroides fragilis. / Liu, Yanlei; Lam, Kan; Tang, Yajarayma J.; Gumerlock, Paul H.; Lee, Dong Ki; Kim, Myung Hwa; Lee, Sum P.; Silva, Joseph; Leung, Joseph.

In: Chinese Medical Journal, Vol. 113, No. 9, 09.2000, p. 858-861.

Research output: Contribution to journalArticle

Liu, Y, Lam, K, Tang, YJ, Gumerlock, PH, Lee, DK, Kim, MH, Lee, SP, Silva, J & Leung, J 2000, 'Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis', Chinese Medical Journal, vol. 113, no. 9, pp. 858-861.
Liu Y, Lam K, Tang YJ, Gumerlock PH, Lee DK, Kim MH et al. Anaerobic bacteria and intrahepatic stones: Detections of Clostridium sp. and Bacteroides fragilis. Chinese Medical Journal. 2000 Sep;113(9):858-861.
Liu, Yanlei ; Lam, Kan ; Tang, Yajarayma J. ; Gumerlock, Paul H. ; Lee, Dong Ki ; Kim, Myung Hwa ; Lee, Sum P. ; Silva, Joseph ; Leung, Joseph. / Anaerobic bacteria and intrahepatic stones : Detections of Clostridium sp. and Bacteroides fragilis. In: Chinese Medical Journal. 2000 ; Vol. 113, No. 9. pp. 858-861.
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abstract = "Objective: To detect anaerobic bacteria Clostridium sp. and Bacteroides fragilis in intrahepatic stones by molecular genetic method. Methods: DNA was extracted from 59 stone samples and subjected to polymerase chain reaction (PCR) amplification targeting the 16S rRNA gene of Clostridium sp. and the glutamine synthetase gene of Bacteroides fragilis. Single-strand conformational polymorphism (SSCP) analysis was performed to identify the Clostridium sp. Results: 16S rRNA gene sequences for Clostridium sp. were identified in 49 stones (83{\%}, 49/59). The two most common groups were detected in 19 (41{\%}) and 17 (37{\%}) of the 46 samples using SSPC analysis, and 25/59 (42{\%}) stones were tested positive for Bacteroides fragilis. Conclusions: Anaerobes such as Clostridium sp. and Bacteroides fragilis present in intrahepatic stones and may play a role in stone formation. PCR is a useful technique to detect fastidious pathogens, which are difficult to culture. SSCP of PCR products is a rapid method in differentiating bacterial species.",
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