Ana o 1, a cashew (Anacardium occidental) allergen of the vicilin seed storage protein family

Fang Wang, Jason M. Robotham, Suzanne S Teuber, Pallavi Tawde, Shridhar K. Sathe, Kenneth H. Roux

Research output: Contribution to journalArticlepeer-review

138 Scopus citations


Background: The allergens responsible for cashew food allergy have not been well characterized. Objectives: We initiated a study to clone cDNAs encoding cashew food allergens. Methods: A cashew cDNA library was screened with human serum for IgE-reactive clones and rabbit IgG anti-cashew extract antisera. Reactive clones were sequenced and expressed, and linear epitopes were identified by means of solid-phase overlapping peptide analysis. Immunoblot inhibition was used to identify the native peptide in cashew extract. Results: Four closely related clones reactive with both human and rabbit antisera were sequenced. Sequence analysis showed that these encode members of the vicilin/sucrose-binding protein family of plant seed storage proteins. Screening of the recombinant protein with sera from 20 patients with cashew allergy and 8 cashew-tolerant patients with allergies to other tree nuts showed that 50% and 25% of sera from patients with cashew allergy and cashew-tolerant subjects, respectively, bound the recombinant protein. The corresponding native allergen protein, designated Ana o 1, was located at approximately 50 kd. Epitope mapping revealed 11 linear IgE-binding epitopes, of which 3 appear to be immunodominant. None of the epitopes were shared in common with those of the peanut vicilin allergen Ara h 1. Conclusion: Ana o 1, a vicilin-like protein, is a major food allergen in cashews. Cashew and peanut vicilins do not share linear epitopes.

Original languageEnglish (US)
Pages (from-to)160-166
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Issue number1
StatePublished - 2002


  • 7S globulin
  • Ana o 1
  • Anacardium occidental
  • Cashew allergy
  • Epitope map
  • Food allergy
  • Linear epitope
  • Seed storage proteins
  • Sucrose-binding protein
  • Tree nut allergy
  • Vicilin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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