An RTX operon in hemolytic Moraxella bovis is absent from nonhemolytic strains

John A Angelos, John F. Hess, Lisle W. George

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Pathogenic isolates of Moraxella bovis express a calcium-dependent transmembrane pore forming cytotoxin that is an RTX toxin encoded by mbxA. The DNA flanking mbxA was cloned and sequenced to determine if M. bovis contained a classical RTX operon. Open reading frames (ORFs) with deduced amino acid sequence homology to putative activation (RTX C) and transport (RTX B and D) proteins were identified and have been designated MbxC, MbxB, and MbxD, respectively. Thus, hemolytic M. bovis contains a typical RTX operon comprised of four genes arranged (5′-3′) mbxCABD. In addition, the deduced amino acid sequences of DNA flanking mbxCABD revealed ORFs with amino acid sequence similarity to transposases (5′). At the 3′ end of the mbx gene cluster, an ORF with homology to bacterial tolC genes was identified. Thus, as with the cya RTX operon of Bordetella pertussis, M. bovis appears to have a secretion accessory protein linked to RTX genes. Analysis of genomic DNA isolated from 5 nonhemolytic M. bovis strains by PCR and Southern blotting revealed the absence of mbxCABD. These strains did, however, amplify with primers specific for the 5′ region flanking mbxC. M. bovis harbors a classical RTX operon that is absent in nonhemolytic strains.

Original languageEnglish (US)
Pages (from-to)363-377
Number of pages15
JournalVeterinary Microbiology
Volume92
Issue number4
DOIs
StatePublished - Apr 29 2003

Fingerprint

Moraxella (Moraxella) bovis
Moraxella bovis
operon
Operon
Open Reading Frames
open reading frames
amino acid sequences
Amino Acid Sequence
DNA
Bordetella pertussis
Transposases
cytotoxins
Bacterial Genes
Amino Acid Sequence Homology
genes
5' Flanking Region
Cytotoxins
Multigene Family
Southern Blotting
sequence homology

Keywords

  • Cytotoxin
  • Infectious bovine keratoconjunctivitis
  • Moraxella bovis
  • RTX

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

An RTX operon in hemolytic Moraxella bovis is absent from nonhemolytic strains. / Angelos, John A; Hess, John F.; George, Lisle W.

In: Veterinary Microbiology, Vol. 92, No. 4, 29.04.2003, p. 363-377.

Research output: Contribution to journalArticle

Angelos, John A ; Hess, John F. ; George, Lisle W. / An RTX operon in hemolytic Moraxella bovis is absent from nonhemolytic strains. In: Veterinary Microbiology. 2003 ; Vol. 92, No. 4. pp. 363-377.
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