An Outer Segment Localization Signal at the C Terminus of the Photoreceptor-Specific Retinol Dehydrogenase

Wenqin Luo, Nicholas Marsh-Armstrong, Amir Rattner, Jeremy Nathans

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Photoreceptor retinol dehydrogenase (prRDH) is a membrane-associated cytosolic protein that localizes to the outer segments (OS) of rods and cones. Here, we demonstrate that the C-terminal 16 amino acids of prRDH confer membrane association as well as cone and rod OS targeting on a linked green fluorescent protein. Membrane association in transfected 293 cells and in transgenic Xenopus photoreceptors is mediated by fatty acylation at one or more evolutionarily conserved cysteines within the prRDH C-terminal tail. In bovine OS, native prRDH is similarly acylated, and hydrolysis of this linkage releases the protein from the membrane. Efficient OS localization requires both membrane association and the prRDH sequence ... (V/I)XPX at the extreme C terminus, which closely resembles the C-terminal sequence that targets opsin/rhodopsin to the OS. Taken together, these data imply that the C-terminal ... (V/I)XPX sequence is a general OS localization signal that can function in the context of both integral and peripheral membrane proteins. This strategy for OS localization resembles those used for protein localization to mitochondria, peroxisomes, endosomes, and endoplasmic reticulum; in each case, a short N- or C-terminal sequence is shared among structurally diverse proteins that are targeted to the same subcellular destination.

Original languageEnglish (US)
Pages (from-to)2623-2632
Number of pages10
JournalJournal of Neuroscience
Volume24
Issue number11
DOIs
StatePublished - Mar 17 2004
Externally publishedYes

Fingerprint

Membrane Proteins
Membranes
Opsins
Rod Cell Outer Segment
Vertebrate Photoreceptor Cells
Peroxisomes
Rhodopsin
Acylation
Endosomes
Xenopus
Green Fluorescent Proteins
Endoplasmic Reticulum
Cysteine
Tail
Mitochondria
Proteins
Hydrolysis
retinol dehydrogenase
Amino Acids

Keywords

  • Inner segment
  • Outer segment
  • Palmitoylation
  • Photoreceptor
  • Protein sorting
  • Retinol dehydrogenase
  • Transgenic Xenopus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

An Outer Segment Localization Signal at the C Terminus of the Photoreceptor-Specific Retinol Dehydrogenase. / Luo, Wenqin; Marsh-Armstrong, Nicholas; Rattner, Amir; Nathans, Jeremy.

In: Journal of Neuroscience, Vol. 24, No. 11, 17.03.2004, p. 2623-2632.

Research output: Contribution to journalArticle

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