An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support

Yiyan Fei, James P. Landry, Yanhong Li, Hai Yu, Kam Lau, Shengshu Huang, Harshal A. Chokhawala, Xi Chen, X. D. Zhu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A biological state is equilibrium of multiple concurrent biomolecular reactions. The relative importance of these reactions depends on physiological temperature typically between 10 °C and 50 °C. Experimentally the temperature dependence of binding reaction constants reveals thermodynamics and thus details of these biomolecular processes. We developed a variable-temperature opto-fluidic system for real-time measurement of multiple (400-10 000) biomolecular binding reactions on solid supports from 10 °C to 60 °C within ±0.1 °C. We illustrate the performance of this system with investigation of binding reactions of plant lectins (carbohydrate-binding proteins) with 24 synthetic glycans (i.e., carbohydrates). We found that the lectin-glycan reactions in general can be enthalpy-driven, entropy-driven, or both, and water molecules play critical roles in the thermodynamics of these reactions.

Original languageEnglish (US)
Article number114102
JournalReview of Scientific Instruments
Volume84
Issue number11
DOIs
StatePublished - Nov 2013

ASJC Scopus subject areas

  • Instrumentation
  • Medicine(all)

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