Abstract
The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 24403-24413 |
| Number of pages | 11 |
| Journal | Optics Express |
| Volume | 17 |
| Issue number | 26 |
| DOIs | |
| State | Published - Dec 21 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
An optical imaging method to monitor stem cell migration in a model of immune-mediated arthritis. / Sutton, Elizabeth J.; Boddington, Sophie E.; Nedopil, Alexander J.; Henning, Tobias D.; Demos, Stavros G.; Baehner, Rick; Sennino, Barbara; Lu, Ying; Daldrup-Link, Heike E.
In: Optics Express, Vol. 17, No. 26, 21.12.2009, p. 24403-24413.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An optical imaging method to monitor stem cell migration in a model of immune-mediated arthritis
AU - Sutton, Elizabeth J.
AU - Boddington, Sophie E.
AU - Nedopil, Alexander J.
AU - Henning, Tobias D.
AU - Demos, Stavros G.
AU - Baehner, Rick
AU - Sennino, Barbara
AU - Lu, Ying
AU - Daldrup-Link, Heike E.
PY - 2009/12/21
Y1 - 2009/12/21
N2 - The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.
AB - The objective of this work is to establish an optical imaging technique that would enable monitoring of the integration of mesenchymal stem cells (MSC) in arthritic joints. Our approach is based on first developing a labeling technique of MSC with the fluorescent dye DiD followed by tracking the cell migration kinetics from the spatial distribution of the DiD fluorescence in optical images (OI). The experimental approach involves first the in vitro OI of MSC labeled with DiD accompanied by fluorescence microscopy measurements to establish localization of the signal within the cells. Thereafter, DiD-labeled MSC were injected into polyarthritic, athymic rats and the signal localization within the experimental animals was monitored over several days. The experimental results indicate that DiD integrated into the cell membrane. DiD-labeled MSC localization in the arthritic ankle joints was observed with OI indicating that this method can be applied to monitor MSC in arthritic joints.
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U2 - 10.1364/OE.17.024403
DO - 10.1364/OE.17.024403
M3 - Article
VL - 17
SP - 24403
EP - 24413
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 26
ER -