An Mtb-Human Protein-Protein Interaction Map Identifies a Switch between Host Antiviral and Antibacterial Responses

Bennett Penn, Zoe Netter, Jeffrey R. Johnson, John Von Dollen, Gwendolyn M. Jang, Tasha Johnson, Yamini M. Ohol, Cyrus Maher, Samantha L. Bell, Kristina Geiger, Guillaume Golovkine, Xiaotang Du, Alex Choi, Trevor Parry, Bhopal C. Mohapatra, Matthew D. Storck, Hamid Band, Chen Chen, Stefanie Jäger, Michael ShalesDan A. Portnoy, Ryan Hernandez, Laurent Coscoy, Jeffery S. Cox, Nevan J. Krogan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although macrophages are armed with potent antibacterial functions, Mycobacterium tuberculosis (Mtb) replicates inside these innate immune cells. Determinants of macrophage intrinsic bacterial control, and the Mtb strategies to overcome them, are poorly understood. To further study these processes, we used an affinity tag purification mass spectrometry (AP-MS) approach to identify 187 Mtb-human protein-protein interactions (PPIs) involving 34 secreted Mtb proteins. This interaction map revealed two factors involved in Mtb pathogenesis—the secreted Mtb protein, LpqN, and its binding partner, the human ubiquitin ligase CBL. We discovered that an lpqN Mtb mutant is attenuated in macrophages, but growth is restored when CBL is removed. Conversely, Cbl−/− macrophages are resistant to viral infection, indicating that CBL regulates cell-intrinsic polarization between antibacterial and antiviral immunity. Collectively, these findings illustrate the utility of this Mtb-human PPI map for developing a deeper understanding of the intricate interactions between Mtb and its host. Penn et al. used an affinity tag purification mass spectrometry approach to generate an Mtb-human protein-protein interaction map, uncovering a connection between LpqN, a virulence factor in Mtb, and CBL, a host ubiquitin ligase. CBL suppresses lpqN attenuation and acts as a switch for host antibacterial and antiviral responses.

Original languageEnglish (US)
Pages (from-to)637-648.e5
JournalMolecular Cell
Volume71
Issue number4
DOIs
StatePublished - Aug 16 2018

Fingerprint

Protein Interaction Maps
Mycobacterium tuberculosis
Antiviral Agents
Macrophages
Ligases
Ubiquitin
Mass Spectrometry
Proteins
Virulence Factors
Virus Diseases
Immunity

Keywords

  • Cbl
  • host-pathogen interaction
  • LpqN
  • macrophage
  • mycobacterium
  • protein-protein interaction
  • tuberculosis
  • ubiquitin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Penn, B., Netter, Z., Johnson, J. R., Von Dollen, J., Jang, G. M., Johnson, T., ... Krogan, N. J. (2018). An Mtb-Human Protein-Protein Interaction Map Identifies a Switch between Host Antiviral and Antibacterial Responses. Molecular Cell, 71(4), 637-648.e5. https://doi.org/10.1016/j.molcel.2018.07.010

An Mtb-Human Protein-Protein Interaction Map Identifies a Switch between Host Antiviral and Antibacterial Responses. / Penn, Bennett; Netter, Zoe; Johnson, Jeffrey R.; Von Dollen, John; Jang, Gwendolyn M.; Johnson, Tasha; Ohol, Yamini M.; Maher, Cyrus; Bell, Samantha L.; Geiger, Kristina; Golovkine, Guillaume; Du, Xiaotang; Choi, Alex; Parry, Trevor; Mohapatra, Bhopal C.; Storck, Matthew D.; Band, Hamid; Chen, Chen; Jäger, Stefanie; Shales, Michael; Portnoy, Dan A.; Hernandez, Ryan; Coscoy, Laurent; Cox, Jeffery S.; Krogan, Nevan J.

In: Molecular Cell, Vol. 71, No. 4, 16.08.2018, p. 637-648.e5.

Research output: Contribution to journalArticle

Penn, B, Netter, Z, Johnson, JR, Von Dollen, J, Jang, GM, Johnson, T, Ohol, YM, Maher, C, Bell, SL, Geiger, K, Golovkine, G, Du, X, Choi, A, Parry, T, Mohapatra, BC, Storck, MD, Band, H, Chen, C, Jäger, S, Shales, M, Portnoy, DA, Hernandez, R, Coscoy, L, Cox, JS & Krogan, NJ 2018, 'An Mtb-Human Protein-Protein Interaction Map Identifies a Switch between Host Antiviral and Antibacterial Responses', Molecular Cell, vol. 71, no. 4, pp. 637-648.e5. https://doi.org/10.1016/j.molcel.2018.07.010
Penn, Bennett ; Netter, Zoe ; Johnson, Jeffrey R. ; Von Dollen, John ; Jang, Gwendolyn M. ; Johnson, Tasha ; Ohol, Yamini M. ; Maher, Cyrus ; Bell, Samantha L. ; Geiger, Kristina ; Golovkine, Guillaume ; Du, Xiaotang ; Choi, Alex ; Parry, Trevor ; Mohapatra, Bhopal C. ; Storck, Matthew D. ; Band, Hamid ; Chen, Chen ; Jäger, Stefanie ; Shales, Michael ; Portnoy, Dan A. ; Hernandez, Ryan ; Coscoy, Laurent ; Cox, Jeffery S. ; Krogan, Nevan J. / An Mtb-Human Protein-Protein Interaction Map Identifies a Switch between Host Antiviral and Antibacterial Responses. In: Molecular Cell. 2018 ; Vol. 71, No. 4. pp. 637-648.e5.
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