Abstract
We describe the moving wire interface attached to the 1-MV AMS system at LLNL's Center for Accelerator Mass Spectrometry for the analysis of nonvolatile liquid samples as either discrete drops or from the direct output of biochemical separatory instrumentation, such as high-performance liquid chromatography (HPLC). Discrete samples containing at least a few 10 s of nanograms of carbon and as little as 50 zmol 14C can be measured with a 3-5% precision in a few minutes. The dynamic range of our system spans approximately 3 orders in magnitude. Sample to sample memory is minimized by the use of fresh targets for each discrete sample or by minimizing the amount of carbon present in a peak generated by an HPLC containing a significant amount of 14C. Liquid sample AMS provides a new technology to expand our biomedical AMS program by enabling the capability to measure low-level biochemicals in extremely small samples that would otherwise be inaccessible.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 173-177 |
| Number of pages | 5 |
| Journal | Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms |
| Volume | 361 |
| DOIs | |
| State | Published - Oct 15 2015 |
| Externally published | Yes |
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Keywords
- C
- Accelerator Mass Spectrometry
- CO
- HPLC interfaces
ASJC Scopus subject areas
- Instrumentation
- Nuclear and High Energy Physics
Cite this
An interface for the direct coupling of small liquid samples to AMS. / Ognibene, T. J.; Thomas, A. T.; Daley, P. F.; Bench, G.; Turteltaub, Ken W.
In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 361, 15.10.2015, p. 173-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An interface for the direct coupling of small liquid samples to AMS
AU - Ognibene, T. J.
AU - Thomas, A. T.
AU - Daley, P. F.
AU - Bench, G.
AU - Turteltaub, Ken W
PY - 2015/10/15
Y1 - 2015/10/15
N2 - We describe the moving wire interface attached to the 1-MV AMS system at LLNL's Center for Accelerator Mass Spectrometry for the analysis of nonvolatile liquid samples as either discrete drops or from the direct output of biochemical separatory instrumentation, such as high-performance liquid chromatography (HPLC). Discrete samples containing at least a few 10 s of nanograms of carbon and as little as 50 zmol 14C can be measured with a 3-5% precision in a few minutes. The dynamic range of our system spans approximately 3 orders in magnitude. Sample to sample memory is minimized by the use of fresh targets for each discrete sample or by minimizing the amount of carbon present in a peak generated by an HPLC containing a significant amount of 14C. Liquid sample AMS provides a new technology to expand our biomedical AMS program by enabling the capability to measure low-level biochemicals in extremely small samples that would otherwise be inaccessible.
AB - We describe the moving wire interface attached to the 1-MV AMS system at LLNL's Center for Accelerator Mass Spectrometry for the analysis of nonvolatile liquid samples as either discrete drops or from the direct output of biochemical separatory instrumentation, such as high-performance liquid chromatography (HPLC). Discrete samples containing at least a few 10 s of nanograms of carbon and as little as 50 zmol 14C can be measured with a 3-5% precision in a few minutes. The dynamic range of our system spans approximately 3 orders in magnitude. Sample to sample memory is minimized by the use of fresh targets for each discrete sample or by minimizing the amount of carbon present in a peak generated by an HPLC containing a significant amount of 14C. Liquid sample AMS provides a new technology to expand our biomedical AMS program by enabling the capability to measure low-level biochemicals in extremely small samples that would otherwise be inaccessible.
KW - C
KW - Accelerator Mass Spectrometry
KW - CO
KW - HPLC interfaces
UR - http://www.scopus.com/inward/record.url?scp=84943584285&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943584285&partnerID=8YFLogxK
U2 - 10.1016/j.nimb.2015.05.018
DO - 10.1016/j.nimb.2015.05.018
M3 - Article
VL - 361
SP - 173
EP - 177
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
SN - 0168-583X
ER -