Abstract
New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware solution to integrate the Ettan two-dimensional difference gel electrophoresis (2-D DIGE) system (GE Healthcare) with the Investigator ProPic spot picking robot (Genomic Solutions). We have analyzed protein sample preparations from bacterial and mammalian sources to demonstrate a new workflow with increased throughput for gel-based proteomics.
Original language | English (US) |
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Pages (from-to) | 2093-2097 |
Number of pages | 5 |
Journal | Journal of Proteome Research |
Volume | 5 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2006 |
Externally published | Yes |
Keywords
- 2-D DIGE
- CyDye
- Gel-based proteomics
- High-throughput
- Integration
- Minimal labeling
- ProPic Workstation
- Proteome
- Saturation labeling
- Yersinia pestis
ASJC Scopus subject areas
- Genetics
- Biotechnology
- Biochemistry