An integrated proteomic workflow for two-dimensional differential gel electrophoresis and robotic spot picking

Ryan C. Mahnke, Todd H. Corzett, Sandra L. McCutchen-Maloney, Brett A. Chromy

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware solution to integrate the Ettan two-dimensional difference gel electrophoresis (2-D DIGE) system (GE Healthcare) with the Investigator ProPic spot picking robot (Genomic Solutions). We have analyzed protein sample preparations from bacterial and mammalian sources to demonstrate a new workflow with increased throughput for gel-based proteomics.

Original languageEnglish (US)
Pages (from-to)2093-2097
Number of pages5
JournalJournal of Proteome Research
Volume5
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

Keywords

  • 2-D DIGE
  • CyDye
  • Gel-based proteomics
  • High-throughput
  • Integration
  • Minimal labeling
  • ProPic Workstation
  • Proteome
  • Saturation labeling
  • Yersinia pestis

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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