An integrated proteomic workflow for two-dimensional differential gel electrophoresis and robotic spot picking

Ryan C. Mahnke; Todd H. Corzett; Sandra L. McCutchen-Maloney; Brett A. Chromy

doi:10.1021/pr050465u

An integrated proteomic workflow for two-dimensional differential gel electrophoresis and robotic spot picking

Ryan C. Mahnke, Todd H. Corzett, Sandra L. McCutchen-Maloney, Brett A. Chromy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

New technologies have advanced the field of proteomics, and a number of companies have developed innovative platforms to drive this research. However, significant challenges are often encountered when trying to integrate complementary technologies from multiple manufacturers. We have developed a software and hardware solution to integrate the Ettan two-dimensional difference gel electrophoresis (2-D DIGE) system (GE Healthcare) with the Investigator ProPic spot picking robot (Genomic Solutions). We have analyzed protein sample preparations from bacterial and mammalian sources to demonstrate a new workflow with increased throughput for gel-based proteomics.

Original language	English (US)
Pages (from-to)	2093-2097
Number of pages	5
Journal	Journal of Proteome Research
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/pr050465u
State	Published - Sep 2006
Externally published	Yes

Keywords

2-D DIGE
CyDye
Gel-based proteomics
High-throughput
Integration
Minimal labeling
ProPic Workstation
Proteome
Saturation labeling
Yersinia pestis

ASJC Scopus subject areas

Genetics
Biotechnology
Biochemistry

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AU - Corzett, Todd H.

AU - McCutchen-Maloney, Sandra L.

AU - Chromy, Brett A.

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KW - Gel-based proteomics

KW - High-throughput

KW - Integration

KW - Minimal labeling

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KW - Proteome

KW - Saturation labeling

KW - Yersinia pestis

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An integrated proteomic workflow for two-dimensional differential gel electrophoresis and robotic spot picking

Abstract

Keywords

ASJC Scopus subject areas

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