An integrated optofluidic platform for Raman-activated cell sorting

Adrian Y. Lau, Luke P. Lee, James W Chan

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

We report on integrated optofluidic Raman-activated cell sorting (RACS) platforms that combine multichannel microfluidic devices and laser tweezers Raman spectroscopy (LTRS) for delivery, identification, and simultaneous sorting of individual cells. The system allows label-free cell identification based on Raman spectroscopy and automated continuous cell sorting. Two optofluidic designs using hydrodynamic focusing and pinch-flow fractionation are evaluated based on their sorting design and flow velocity effect on the laser trapping efficiency at different laser power levels. A proof-of-principle demonstration of the integrated optofluidic LTRS system for the identification and sorting of two leukemia cell lines is presented. This functional prototype lays the foundation for the development of a label-free cell sorting platform based on intrinsic Raman markers for automated sampling and sorting of a large number of individual cells in solution.

Original languageEnglish (US)
Pages (from-to)1116-1120
Number of pages5
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume8
Issue number7
DOIs
StatePublished - 2008

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Sorting
Cells
Raman Spectrum Analysis
Optical Tweezers
Lab-On-A-Chip Devices
Raman spectroscopy
Lasers
Labels
Hydrodynamics
Leukemia
Cell Count
Fractionation
Microfluidics
Flow velocity
Cell Line
Demonstrations
Sampling

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

An integrated optofluidic platform for Raman-activated cell sorting. / Lau, Adrian Y.; Lee, Luke P.; Chan, James W.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 8, No. 7, 2008, p. 1116-1120.

Research output: Contribution to journalArticle

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