An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides

David A. Sela, Yanhong Li, Larry Lerno, Shuai Wu, Angela M. Marcobal, J. Bruce German, Xi Chen, Carlito B Lebrilla, David A. Mills

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Lactating mothers secrete milk sialyloligosaccharides (MSOs) that function as anti-adhesives once provided to the neonate. Particular infant-associated commensals, such as Bifidobacterium longum subsp. infantis, consume neutral milk oligosaccharides, although their ability to utilize acidic oligosaccharides has not been assessed. Temporal glycoprofiling of acidic HMO consumed during fermentation demonstrated a single composition, with several isomers, corresponding to sialylated lacto-Ntetraose. To utilize MSO, B. longum subsp. infantis deploys a sialidase that cleaves α2-6 and α2-3 linkages. NanH2, encoded within the HMO catabolic cluster is up-regulated during HMO fermentation and is active on sialylated lacto-N-tetraose. These results demonstrate that commensal microorganisms do utilize MSO, a substrate that may be enriched in the distal gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)11909-11918
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number14
DOIs
StatePublished - Apr 8 2011

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Human Milk
Health Maintenance Organizations
Milk
Oligosaccharides
Fermentation
Aptitude
Neuraminidase
Isomers
Microorganisms
Adhesives
Gastrointestinal Tract
Mothers
Newborn Infant
sialooligosaccharides
Substrates
Chemical analysis
Bifidobacterium longum

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Sela, D. A., Li, Y., Lerno, L., Wu, S., Marcobal, A. M., Bruce German, J., ... Mills, D. A. (2011). An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides. Journal of Biological Chemistry, 286(14), 11909-11918. https://doi.org/10.1074/jbc.M110.193359

An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides. / Sela, David A.; Li, Yanhong; Lerno, Larry; Wu, Shuai; Marcobal, Angela M.; Bruce German, J.; Chen, Xi; Lebrilla, Carlito B; Mills, David A.

In: Journal of Biological Chemistry, Vol. 286, No. 14, 08.04.2011, p. 11909-11918.

Research output: Contribution to journalArticle

Sela, DA, Li, Y, Lerno, L, Wu, S, Marcobal, AM, Bruce German, J, Chen, X, Lebrilla, CB & Mills, DA 2011, 'An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides', Journal of Biological Chemistry, vol. 286, no. 14, pp. 11909-11918. https://doi.org/10.1074/jbc.M110.193359
Sela, David A. ; Li, Yanhong ; Lerno, Larry ; Wu, Shuai ; Marcobal, Angela M. ; Bruce German, J. ; Chen, Xi ; Lebrilla, Carlito B ; Mills, David A. / An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 14. pp. 11909-11918.
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