An indispensable gene for NAD biosynthesis in Salmonella typhimurium

K. T. Hughes, D. Ladika, J. R. Roth, B. M. Olivera

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

We have located the nadD locus between lip and leuS at 14 min on the Salmonella typhimurium chromosome, and we have shown it to be the structural gene for nicotinic acid mononucleotide adenylyltransferase. This is the first indispensable gene of pyridine nucleotide metabolism that has been identified. Mutants altered at this locus, isolated by their 6-aminonicotinamide resistance phenotype, accumulate abnormally large pools of nicotinic acid mononucleotide in vivo; many exhibit a temperature-sensitive lethal phenotype. Enzyme assays reveal markedly lower transferase activity in mutant extracts than in nadD+ extracts. The partial dominance of nadD mutants when placed in a nadD+/nadD diploid suggests that nicotinic acid mononucleotide adenylyltransferase is a multimeric enzyme.

Original languageEnglish (US)
Pages (from-to)213-221
Number of pages9
JournalJournal of Bacteriology
Volume155
Issue number1
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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    Hughes, K. T., Ladika, D., Roth, J. R., & Olivera, B. M. (1983). An indispensable gene for NAD biosynthesis in Salmonella typhimurium. Journal of Bacteriology, 155(1), 213-221.