An indirect enzyme-linked immunosorbent assay for diagnosis of American canine hepatozoonosis

John S. Mathew, Jeremiah T. Saliki, Sidney A. Ewing, Terry W Lehenbauer, Roger J. Panciera, Jerry R. Malayer, Connie A. Cummings, A. Alan Kocan

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


American canine hepatozoonosis (ACH), caused by Hepatozoon americanum, is an emerging tick-borne disease of dogs. An indirect enzyme-linked immunosorbent assay (ELISA) that should facilitate diagnosis of infection and study of the epidemiology of ACH has been developed using H. americanum sporozoites as antigen. Efficacy of the new test as a diagnostic tool was compared with that of skeletal muscle biopsy, the current gold standard for confirming H. americanum infection. Results show that the test is sensitive (93%) and specific (96%) and that it is as reliable as histopathologic examination of skeletal muscle for detecting infection. The ELISA would be suitable as a routine laboratory test for diagnosis of ACH.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Issue number1
StatePublished - Jan 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)


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