An indirect ELISA using a monoclonal anti IgG1 enzyme conjugate for the diagnosis of bovine brucellosis

Francisco A Uzal, A. E. Carrasco, K. Nielsen, S. Echaide, R. F. Cabrera

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An indirect ELISA using a monoclonal anti-IgG1 enzyme conjugate for diagnosis of bovine brucellosis was evaluated. Three groups of sera from negative non-vaccinated, from negative vaccinated and from positive cows were used. The specificity of the ELISA for negative non-vaccinated cows was 99.2 and 99.6% for negative but vaccinated cows. The sensitivity of this technique estimated with positive sera was 99.5%. The ELISA evaluated appears to be a very useful tool in the diagnosis of bovine brucellosis, particularly for regions where little epidemiological information is available about this disease and where large numbers of sera should be tested to obtain such information.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalVeterinary Microbiology
Volume52
Issue number1-2
DOIs
StatePublished - Sep 1996
Externally publishedYes

Fingerprint

Bovine Brucellosis
bovine brucellosis
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
cows
Enzymes
enzymes
Serum
methodology

Keywords

  • Brucella abortus
  • cattle
  • diagnosis-bacteria
  • ELISA
  • monoclonal conjugate

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

An indirect ELISA using a monoclonal anti IgG1 enzyme conjugate for the diagnosis of bovine brucellosis. / Uzal, Francisco A; Carrasco, A. E.; Nielsen, K.; Echaide, S.; Cabrera, R. F.

In: Veterinary Microbiology, Vol. 52, No. 1-2, 09.1996, p. 175-180.

Research output: Contribution to journalArticle

Uzal, Francisco A ; Carrasco, A. E. ; Nielsen, K. ; Echaide, S. ; Cabrera, R. F. / An indirect ELISA using a monoclonal anti IgG1 enzyme conjugate for the diagnosis of bovine brucellosis. In: Veterinary Microbiology. 1996 ; Vol. 52, No. 1-2. pp. 175-180.
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