Abstract
An indirect ELISA using a monoclonal anti-IgG1 enzyme conjugate for diagnosis of bovine brucellosis was evaluated. Three groups of sera from negative non-vaccinated, from negative vaccinated and from positive cows were used. The specificity of the ELISA for negative non-vaccinated cows was 99.2 and 99.6% for negative but vaccinated cows. The sensitivity of this technique estimated with positive sera was 99.5%. The ELISA evaluated appears to be a very useful tool in the diagnosis of bovine brucellosis, particularly for regions where little epidemiological information is available about this disease and where large numbers of sera should be tested to obtain such information.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 175-180 |
| Number of pages | 6 |
| Journal | Veterinary Microbiology |
| Volume | 52 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Sep 1996 |
| Externally published | Yes |
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Keywords
- Brucella abortus
- cattle
- diagnosis-bacteria
- ELISA
- monoclonal conjugate
ASJC Scopus subject areas
- Animal Science and Zoology
- Microbiology
- veterinary(all)
Cite this
An indirect ELISA using a monoclonal anti IgG1 enzyme conjugate for the diagnosis of bovine brucellosis. / Uzal, Francisco A; Carrasco, A. E.; Nielsen, K.; Echaide, S.; Cabrera, R. F.
In: Veterinary Microbiology, Vol. 52, No. 1-2, 09.1996, p. 175-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An indirect ELISA using a monoclonal anti IgG1 enzyme conjugate for the diagnosis of bovine brucellosis
AU - Uzal, Francisco A
AU - Carrasco, A. E.
AU - Nielsen, K.
AU - Echaide, S.
AU - Cabrera, R. F.
PY - 1996/9
Y1 - 1996/9
N2 - An indirect ELISA using a monoclonal anti-IgG1 enzyme conjugate for diagnosis of bovine brucellosis was evaluated. Three groups of sera from negative non-vaccinated, from negative vaccinated and from positive cows were used. The specificity of the ELISA for negative non-vaccinated cows was 99.2 and 99.6% for negative but vaccinated cows. The sensitivity of this technique estimated with positive sera was 99.5%. The ELISA evaluated appears to be a very useful tool in the diagnosis of bovine brucellosis, particularly for regions where little epidemiological information is available about this disease and where large numbers of sera should be tested to obtain such information.
AB - An indirect ELISA using a monoclonal anti-IgG1 enzyme conjugate for diagnosis of bovine brucellosis was evaluated. Three groups of sera from negative non-vaccinated, from negative vaccinated and from positive cows were used. The specificity of the ELISA for negative non-vaccinated cows was 99.2 and 99.6% for negative but vaccinated cows. The sensitivity of this technique estimated with positive sera was 99.5%. The ELISA evaluated appears to be a very useful tool in the diagnosis of bovine brucellosis, particularly for regions where little epidemiological information is available about this disease and where large numbers of sera should be tested to obtain such information.
KW - Brucella abortus
KW - cattle
KW - diagnosis-bacteria
KW - ELISA
KW - monoclonal conjugate
UR - http://www.scopus.com/inward/record.url?scp=0030250067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030250067&partnerID=8YFLogxK
U2 - 10.1016/0378-1135(96)00026-0
DO - 10.1016/0378-1135(96)00026-0
M3 - Article
C2 - 8914261
AN - SCOPUS:0030250067
VL - 52
SP - 175
EP - 180
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 1-2
ER -