An in vivo method for recording single unit activity in lumbar spinal cord in mice anesthetized with a volatile anesthetic

Jason M. Cuellar, Joseph F. Antognini, Earl Carstens

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We describe a method to record single unit neuronal activity from mouse spinal cord using volatile anesthesia. The small size of the mouse can complicate usual methods that are used for single-unit recording in rats, but simple modifications can significantly increase the number of successful recordings. Stabilization of the vertebral column is particularly important, as are adequate ventilation of the animal, control of body temperature and accurate determination of anesthetic concentrations in respiratory gas samples.

Original languageEnglish (US)
Pages (from-to)126-134
Number of pages9
JournalBrain Research Protocols
Volume13
Issue number2
DOIs
StatePublished - Jun 2004

Keywords

  • Electrophysiology
  • Halothane
  • In vivo
  • Isoflurane
  • Mouse
  • Sensory systems
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

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