An in vivo evaluation of a chondroitin sulfate solution to prevent postoperative intraperitoneal adhesion formation

H. S. Tran, F. A. Chrzanowski, M. M. Puc, N. G. Patel, B. Geldziler, D. Malli, D. B. Soll, R. Ramsamooj, C. W. Hewitt, A. J. DelRossi

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12 Scopus citations

Abstract

Purpose. The goal of this study was to determine the efficacy of a single intraperitoneal administration of a chondroitin sulfate solution in preventing postoperative adhesion formation. Methods. Twenty-five Sprague- Dawley rats had a 1-cm2 area of cecal serosa abraded. Controls (CON, n = 5) received no treatment, the chondroitin sulfate group (CS, n = 10) received chondroitin sulfate (0.013 g/kg) in 0.9% NaCl intraperitoneally (ip), and vehicle controls (VC, n = 10) received an equal volume of 0.9% NaCl solution ip before the abdomen was closed. All animals were sacrificed on postoperative day 10. The extent of adhesion was quantified according to Mazuji's adhesion grade (0 to 4: 0 = no adhesion and 4 = very dense adhesion) and quantitated after H and E, trichome, and immunohistochemical staining for fibrin and collagen type I and type III using digital image analysis. Results. The mean Mazuji's adhesion grade in the CON was 4.0 ± 0.0, in the VC 2.60 ± 0.37, and in the CS 1.3 ± 0.42 (P < 0.01 for CS vs CON and P < 0.05 for CS vs VC comparisons). The mean gray-scale intensity (0-255: 0 = dense amount and 255 = none) of adhesion density in the CON was 105.5 ± 5.5, in the VC 125 ± 15.0, and in the CS 178.3 ± 21.0 (P < 0.01 for CS vs CON and P < 0.05 for CS vs VC comparisons). The mean adjusted intensity stain indices (AISI) for fibrin and collagen type I in the CON were 59 ± 17 and 53 ± 19, in the VC 27 ± 3 and 25 ± 7, and in the CS 16 ± 5 and 6 ± 3, respectively (P < 0.05 between CS and CON comparisons). The AISI of collagen type III was not significant among all the groups (P > 0.1). Conclusions. The extent of early postoperative intra-abdominal adhesion formation as determined by gross assessment and from quantitation of fibrin and collagen type I deposition was significantly reduced by a single intraperitoneal administration of a chondroitin sulfate solution. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)78-87
Number of pages10
JournalJournal of Surgical Research
Volume88
Issue number2
DOIs
StatePublished - Feb 2000
Externally publishedYes

Keywords

  • Chondroitin sulfate
  • Collagen type I
  • Collagen type III
  • Digital image analysis
  • Fibrin
  • Postoperative adhesion

ASJC Scopus subject areas

  • Surgery

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    Tran, H. S., Chrzanowski, F. A., Puc, M. M., Patel, N. G., Geldziler, B., Malli, D., Soll, D. B., Ramsamooj, R., Hewitt, C. W., & DelRossi, A. J. (2000). An in vivo evaluation of a chondroitin sulfate solution to prevent postoperative intraperitoneal adhesion formation. Journal of Surgical Research, 88(2), 78-87. https://doi.org/10.1006/jsre.1999.5714