An in vitro study on spontaneous myometrial contractility in the mare during estrus and diestrus

Gaby Hirsbrunner, Martin Reist, Suzana S. Couto, Adrian Steiner, Jack Snyder, Elisabeth Vanleeuwen, Irwin Liu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Uterine smooth muscle specimens were collected from euthanatized mares in estrus and diestrus. Longitudinal and circular specimens were mounted in organ baths and the signals transcribed to a Grass polygraph. After equilibration time and 2 g preload, their physiologic isometric contractility was recorded for a continuous 2.0 h. Area under the curve, frequency and time occupied by contractions were studied. Differences between cycle phases, between muscle layers, and over the recorded time periods were statistically evaluated using linear mixed-effect models. In the mare, physiologic contractility of the uterus decreased significantly over time for all variables evaluated (time as covariate on a continuous scale). For area under the curve, there was a significant effect of muscle layer (longitudinal > circular). For frequency, higher values were recorded in estrus for circular smooth muscle layer, whereas higher values were seen in longitudinal smooth muscle layers during diestrus. In longitudinal layer and in diestrus, more time was occupied by contractions than in circular layer, and in estrus. This study is describing physiologic myometrial motility in the organ bath depending on cycle phase.

Original languageEnglish (US)
Pages (from-to)517-527
Number of pages11
JournalTheriogenology
Volume65
Issue number3
DOIs
StatePublished - Feb 1 2006

Fingerprint

Diestrus
diestrus
Estrus
in vitro studies
smooth muscle
mares
estrus
Smooth Muscle
muscles
Baths
uterus
Area Under Curve
grasses
Muscles
Myometrium
Poaceae
Uterus
In Vitro Techniques

Keywords

  • Cycle phase
  • Mare
  • Myometrium
  • Organ bath
  • Physiology

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Equine
  • Animal Science and Zoology

Cite this

An in vitro study on spontaneous myometrial contractility in the mare during estrus and diestrus. / Hirsbrunner, Gaby; Reist, Martin; Couto, Suzana S.; Steiner, Adrian; Snyder, Jack; Vanleeuwen, Elisabeth; Liu, Irwin.

In: Theriogenology, Vol. 65, No. 3, 01.02.2006, p. 517-527.

Research output: Contribution to journalArticle

Hirsbrunner, G, Reist, M, Couto, SS, Steiner, A, Snyder, J, Vanleeuwen, E & Liu, I 2006, 'An in vitro study on spontaneous myometrial contractility in the mare during estrus and diestrus', Theriogenology, vol. 65, no. 3, pp. 517-527. https://doi.org/10.1016/j.theriogenology.2005.05.048
Hirsbrunner, Gaby ; Reist, Martin ; Couto, Suzana S. ; Steiner, Adrian ; Snyder, Jack ; Vanleeuwen, Elisabeth ; Liu, Irwin. / An in vitro study on spontaneous myometrial contractility in the mare during estrus and diestrus. In: Theriogenology. 2006 ; Vol. 65, No. 3. pp. 517-527.
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