An in vitro rainbow trout cell bioassay for aryl hydrocarbon receptor- mediated toxins

Catherine A. Richter, Virginia L. Tieber, Michael S. Denison, John P. Giesy

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Halogenated aromatic hydrocarbons (HAHs) and other chemicals that act as aryl hydrocarbon (Ah) receptor (AhR) agonists cause a variety of toxic effects. In sac fry of many fish species, these effects include blue-sac disease and mortality. Because HAHs occur in complex mixtures, their toxicity in the environment is difficult to predict. A bioassay useful in predicting AhR-mediated toxicity to fish was developed using the RTH-149 rainbow trout hepatoma cell line. Stable transfection of this cell line with the pGudLuc 1.1 plasmid, which contains a firefly luciferase reporter gene under the transcriptional regulation of dioxin responsive enhancers, has produced a recombinant cell line designated Remodulated Lightning Trout (RLT 2.0). The RLT 2.0 bioassay method detection limit for 2,3,7,8 tetrachlorodibenzo-p- dioxin (TCDD) is 4 pM. The responses of the RLT 2.0 bioassay to TCDD and several HAH congeners closely matched the responses observed in vivo in fish. The RLT 2.0 bioassay can provide an integrative measure of the total AhR- mediated toxic activity of complex mixtures to fish. The assay will be useful in screening environmental extracts, guiding chemical analysis, and interpreting the AhR-mediated mechanism of toxicity.

Original languageEnglish (US)
Pages (from-to)543-550
Number of pages8
JournalEnvironmental Toxicology and Chemistry
Volume16
Issue number3
DOIs
StatePublished - Mar 1997

Keywords

  • Ah receptor
  • Bioassay
  • Fish
  • PCB
  • TCDD

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

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