An in vitro model of intestinal infection reveals a developmentally regulated transcriptome of Toxoplasma sporozoites and a NF-κB-like signature in infected host cells

Pascale S. Guiton, Janelle M. Sagawa, Heather Fritz, John C. Boothroyd

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Toxoplasmosis is a zoonotic infection affecting approximately 30% of the world's human population. After sexual reproduction in the definitive feline host, Toxoplasma oocysts, each containing 8 sporozoites, are shed into the environment where they can go on to infect humans and other warm-blooded intermediate hosts. Here, we use an in vitro model to assess host transcriptomic changes that occur in the earliest stages of such infections. We show that infection of rat intestinal epithelial cells with mature sporozoites primarily results in higher expression of genes associated with Tumor Necrosis Factor alpha (TNFα) signaling via NF-κB. Furthermore, we find that, consistent with their biology, these mature, invaded sporozoites display a transcriptome intermediate between the previously reported day 10 oocysts and that of their tachyzoite counterparts. Thus, this study uncovers novel host and pathogen factors that may be critical for the establishment of a successful intracellular niche following sporozoite-initiated infection.

Original languageEnglish (US)
Article numbere0173018
JournalPLoS One
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2017
Externally publishedYes

Fingerprint

Sporozoites
Toxoplasma
sporozoites
Pathogens
Transcriptome
transcriptome
Rats
Tumor Necrosis Factor-alpha
Genes
Oocysts
Infection
oocysts
infection
cells
tachyzoites
toxoplasmosis
Toxoplasmosis
Felidae
Zoonoses
intermediate hosts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

An in vitro model of intestinal infection reveals a developmentally regulated transcriptome of Toxoplasma sporozoites and a NF-κB-like signature in infected host cells. / Guiton, Pascale S.; Sagawa, Janelle M.; Fritz, Heather; Boothroyd, John C.

In: PLoS One, Vol. 12, No. 3, e0173018, 01.03.2017.

Research output: Contribution to journalArticle

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