An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping

Viswanathan V Krishnan, Kevin H. Thornton, Monique Cosman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An improved nuclear magnetic resonance (NMR) experimental scheme to measure self-diffusion coefficients of biomolecules in 95% H2O using pulsed-magnetic-field gradients is presented. The radiation damping of water, which deleteriously effects the quality of the spectra in high-field NMR, is advantageously used in reducing the water signal. The enhancements of the new sequence, designated BPP-SED, a novel combination of BPP-LED (bipolar-gradient-pulse pair longitudinal eddy current delay) and SED (selective echo dephasing) is demonstrated by measuring self-diffusion coefficients of dilute protein samples (0.5 mM) in limited volumes (250 μl).

Original languageEnglish (US)
Pages (from-to)317-323
Number of pages7
JournalChemical Physics Letters
Volume302
Issue number3-4
StatePublished - Mar 19 1999
Externally publishedYes

Fingerprint

Biomolecules
echoes
diffusion coefficient
Damping
damping
Nuclear magnetic resonance
Radiation
gradients
nuclear magnetic resonance
Water
radiation
Eddy currents
eddy currents
water
Magnetic fields
proteins
augmentation
pulses
magnetic fields
Proteins

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping. / Krishnan, Viswanathan V; Thornton, Kevin H.; Cosman, Monique.

In: Chemical Physics Letters, Vol. 302, No. 3-4, 19.03.1999, p. 317-323.

Research output: Contribution to journalArticle

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