Abstract
An improved nuclear magnetic resonance (NMR) experimental scheme to measure self-diffusion coefficients of biomolecules in 95% H2O using pulsed-magnetic-field gradients is presented. The radiation damping of water, which deleteriously effects the quality of the spectra in high-field NMR, is advantageously used in reducing the water signal. The enhancements of the new sequence, designated BPP-SED, a novel combination of BPP-LED (bipolar-gradient-pulse pair longitudinal eddy current delay) and SED (selective echo dephasing) is demonstrated by measuring self-diffusion coefficients of dilute protein samples (0.5 mM) in limited volumes (250 μl).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-323 |
| Number of pages | 7 |
| Journal | Chemical Physics Letters |
| Volume | 302 |
| Issue number | 3-4 |
| State | Published - Mar 19 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Spectroscopy
- Atomic and Molecular Physics, and Optics
Cite this
An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping. / Krishnan, Viswanathan V; Thornton, Kevin H.; Cosman, Monique.
In: Chemical Physics Letters, Vol. 302, No. 3-4, 19.03.1999, p. 317-323.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping
AU - Krishnan, Viswanathan V
AU - Thornton, Kevin H.
AU - Cosman, Monique
PY - 1999/3/19
Y1 - 1999/3/19
N2 - An improved nuclear magnetic resonance (NMR) experimental scheme to measure self-diffusion coefficients of biomolecules in 95% H2O using pulsed-magnetic-field gradients is presented. The radiation damping of water, which deleteriously effects the quality of the spectra in high-field NMR, is advantageously used in reducing the water signal. The enhancements of the new sequence, designated BPP-SED, a novel combination of BPP-LED (bipolar-gradient-pulse pair longitudinal eddy current delay) and SED (selective echo dephasing) is demonstrated by measuring self-diffusion coefficients of dilute protein samples (0.5 mM) in limited volumes (250 μl).
AB - An improved nuclear magnetic resonance (NMR) experimental scheme to measure self-diffusion coefficients of biomolecules in 95% H2O using pulsed-magnetic-field gradients is presented. The radiation damping of water, which deleteriously effects the quality of the spectra in high-field NMR, is advantageously used in reducing the water signal. The enhancements of the new sequence, designated BPP-SED, a novel combination of BPP-LED (bipolar-gradient-pulse pair longitudinal eddy current delay) and SED (selective echo dephasing) is demonstrated by measuring self-diffusion coefficients of dilute protein samples (0.5 mM) in limited volumes (250 μl).
UR - http://www.scopus.com/inward/record.url?scp=0001118712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001118712&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0001118712
VL - 302
SP - 317
EP - 323
JO - Chemical Physics Letters
JF - Chemical Physics Letters
SN - 0009-2614
IS - 3-4
ER -