Abstract
An improved nuclear magnetic resonance (NMR) experimental scheme to measure self-diffusion coefficients of biomolecules in 95% H2O using pulsed-magnetic-field gradients is presented. The radiation damping of water, which deleteriously effects the quality of the spectra in high-field NMR, is advantageously used in reducing the water signal. The enhancements of the new sequence, designated BPP-SED, a novel combination of BPP-LED (bipolar-gradient-pulse pair longitudinal eddy current delay) and SED (selective echo dephasing) is demonstrated by measuring self-diffusion coefficients of dilute protein samples (0.5 mM) in limited volumes (250 μl).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 317-323 |
| Number of pages | 7 |
| Journal | Chemical Physics Letters |
| Volume | 302 |
| Issue number | 3-4 |
| State | Published - Mar 19 1999 |
| Externally published | Yes |
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Spectroscopy
- Atomic and Molecular Physics, and Optics
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Krishnan, V. V., Thornton, K. H., & Cosman, M. (1999). An improved experimental scheme to measure self-diffusion coefficients of biomolecules with an advantageous use of radiation damping. Chemical Physics Letters, 302(3-4), 317-323.