An immunohistochemical panel to assess ultraviolet radiation associated oxidative skin injury

Andrew Mamalis, Natallia Fiadorchanka, Lauren Adams, Melissa Serravallo, Edward Heilman, Daniel Siegel, Neil Brody, Jared Jagdeo

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Ultraviolet (UV) radiation results in a significant loss in years of healthy life, approximately 1.5 million disability-adjusted life years (DALYs), and is associated with greater than 60,000 deaths annually worldwide that are attributed to melanoma and other skin cancers. Currently, there are no standardized biomarkers or assay panels to assess oxidative stress skin injury patterns in human skin exposed to ionizing radiation. Using biopsy specimens from chronic solar UV-exposed and UV-protected skin, we demonstrate that UV radiation-induced oxidative skin injury can be evaluated by an immunohistochemical panel that stains 8-hydroxydeoxyguanosine (8-OH-dG) to assess DNA adducts, 4-hydroxy-2-nonenal (HNE) to assess lipid peroxidation, and advanced glycation end products (AGEs) to assess protein damage. We believe this panel contains the necessary cellular biomarkers to evaluate topical agents, such as sunscreens and anti-oxidants that are designed to prevent oxidative skin damage and may reduce UV-associated skin aging, carcinogenesis, and inflammatory skin diseases. We envision that this panel will become an important tool for researchers developing topical agents to protect against UV radiation and other oxidants and ultimately lead to reductions in lost years of healthy life, DALYs, and annual deaths associated with UV radiation.

Original languageEnglish (US)
Pages (from-to)574-578
Number of pages5
JournalJournal of Drugs in Dermatology
Volume13
Issue number5
StatePublished - 2014

ASJC Scopus subject areas

  • Dermatology
  • Medicine(all)

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  • Cite this

    Mamalis, A., Fiadorchanka, N., Adams, L., Serravallo, M., Heilman, E., Siegel, D., Brody, N., & Jagdeo, J. (2014). An immunohistochemical panel to assess ultraviolet radiation associated oxidative skin injury. Journal of Drugs in Dermatology, 13(5), 574-578.