An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban

Ki Chang Ahn, Takeo Kasagami, Hsing Ju Tsai, Nils Helge Schebb, Temitope Ogunyoku, Shirley J. Gee, Thomas M. Young, Bruce D. Hammock

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclocarban (TCC) was developed. The haptens were synthesized by derivatizing the para position of a phenyl moiety of TCC. The rabbit antisera were screened and the combination of antiserum 1648 and a heterologous competitive hapten containing a piperidine was further characterized. The IC 50 and detection range for TCC in buffer were 0.70 and 0.13-3.60 ng/mL, respectively. The assay was selective for TCC, providing only low cross-reactivity to TCC-related compounds and its major metabolites except for the closely related antimicrobial 3-trifluoromethyl-4, 4′-dichlorocarbanilide. A liquid-liquid extraction for sample preparation of human body fluids resulted in an assay that measured low part per billion levels of TCC in small volumes of the samples. The limits of quantification of TCC were 5 ng/mL in blood/serum and 10 ng/mL in urine, respectively. TCC in human urine was largely the N- or N′-glucuronide. TCC concentrations of biosolids measured by the ELISA were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid, inexpensive, and convenient tool to aid researchers monitoring human/environmental exposure to TCC to better understand the health effects.

Original languageEnglish (US)
Pages (from-to)374-381
Number of pages8
JournalEnvironmental Science and Technology
Volume46
Issue number1
DOIs
StatePublished - Jan 3 2012

Fingerprint

immunoassay
assay
urine
enzyme
Assays
body fluid
liquid
biosolid
sample preparation
Immunosorbents
Haptens
aid
serum
metabolite
blood
Immune Sera
exposure
triclocarban
Biosolids
monitoring

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Ahn, K. C., Kasagami, T., Tsai, H. J., Schebb, N. H., Ogunyoku, T., Gee, S. J., ... Hammock, B. D. (2012). An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban. Environmental Science and Technology, 46(1), 374-381. https://doi.org/10.1021/es202494d

An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban. / Ahn, Ki Chang; Kasagami, Takeo; Tsai, Hsing Ju; Schebb, Nils Helge; Ogunyoku, Temitope; Gee, Shirley J.; Young, Thomas M.; Hammock, Bruce D.

In: Environmental Science and Technology, Vol. 46, No. 1, 03.01.2012, p. 374-381.

Research output: Contribution to journalArticle

Ahn, KC, Kasagami, T, Tsai, HJ, Schebb, NH, Ogunyoku, T, Gee, SJ, Young, TM & Hammock, BD 2012, 'An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban', Environmental Science and Technology, vol. 46, no. 1, pp. 374-381. https://doi.org/10.1021/es202494d
Ahn, Ki Chang ; Kasagami, Takeo ; Tsai, Hsing Ju ; Schebb, Nils Helge ; Ogunyoku, Temitope ; Gee, Shirley J. ; Young, Thomas M. ; Hammock, Bruce D. / An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban. In: Environmental Science and Technology. 2012 ; Vol. 46, No. 1. pp. 374-381.
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