Abstract
A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclocarban (TCC) was developed. The haptens were synthesized by derivatizing the para position of a phenyl moiety of TCC. The rabbit antisera were screened and the combination of antiserum 1648 and a heterologous competitive hapten containing a piperidine was further characterized. The IC 50 and detection range for TCC in buffer were 0.70 and 0.13-3.60 ng/mL, respectively. The assay was selective for TCC, providing only low cross-reactivity to TCC-related compounds and its major metabolites except for the closely related antimicrobial 3-trifluoromethyl-4, 4′-dichlorocarbanilide. A liquid-liquid extraction for sample preparation of human body fluids resulted in an assay that measured low part per billion levels of TCC in small volumes of the samples. The limits of quantification of TCC were 5 ng/mL in blood/serum and 10 ng/mL in urine, respectively. TCC in human urine was largely the N- or N′-glucuronide. TCC concentrations of biosolids measured by the ELISA were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid, inexpensive, and convenient tool to aid researchers monitoring human/environmental exposure to TCC to better understand the health effects.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 374-381 |
| Number of pages | 8 |
| Journal | Environmental Science and Technology |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 3 2012 |
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ASJC Scopus subject areas
- Chemistry(all)
- Environmental Chemistry
Cite this
An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban. / Ahn, Ki Chang; Kasagami, Takeo; Tsai, Hsing Ju; Schebb, Nils Helge; Ogunyoku, Temitope; Gee, Shirley J.; Young, Thomas M.; Hammock, Bruce D.
In: Environmental Science and Technology, Vol. 46, No. 1, 03.01.2012, p. 374-381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An immunoassay to evaluate human/environmental exposure to the antimicrobial triclocarban
AU - Ahn, Ki Chang
AU - Kasagami, Takeo
AU - Tsai, Hsing Ju
AU - Schebb, Nils Helge
AU - Ogunyoku, Temitope
AU - Gee, Shirley J.
AU - Young, Thomas M.
AU - Hammock, Bruce D.
PY - 2012/1/3
Y1 - 2012/1/3
N2 - A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclocarban (TCC) was developed. The haptens were synthesized by derivatizing the para position of a phenyl moiety of TCC. The rabbit antisera were screened and the combination of antiserum 1648 and a heterologous competitive hapten containing a piperidine was further characterized. The IC 50 and detection range for TCC in buffer were 0.70 and 0.13-3.60 ng/mL, respectively. The assay was selective for TCC, providing only low cross-reactivity to TCC-related compounds and its major metabolites except for the closely related antimicrobial 3-trifluoromethyl-4, 4′-dichlorocarbanilide. A liquid-liquid extraction for sample preparation of human body fluids resulted in an assay that measured low part per billion levels of TCC in small volumes of the samples. The limits of quantification of TCC were 5 ng/mL in blood/serum and 10 ng/mL in urine, respectively. TCC in human urine was largely the N- or N′-glucuronide. TCC concentrations of biosolids measured by the ELISA were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid, inexpensive, and convenient tool to aid researchers monitoring human/environmental exposure to TCC to better understand the health effects.
AB - A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclocarban (TCC) was developed. The haptens were synthesized by derivatizing the para position of a phenyl moiety of TCC. The rabbit antisera were screened and the combination of antiserum 1648 and a heterologous competitive hapten containing a piperidine was further characterized. The IC 50 and detection range for TCC in buffer were 0.70 and 0.13-3.60 ng/mL, respectively. The assay was selective for TCC, providing only low cross-reactivity to TCC-related compounds and its major metabolites except for the closely related antimicrobial 3-trifluoromethyl-4, 4′-dichlorocarbanilide. A liquid-liquid extraction for sample preparation of human body fluids resulted in an assay that measured low part per billion levels of TCC in small volumes of the samples. The limits of quantification of TCC were 5 ng/mL in blood/serum and 10 ng/mL in urine, respectively. TCC in human urine was largely the N- or N′-glucuronide. TCC concentrations of biosolids measured by the ELISA were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid, inexpensive, and convenient tool to aid researchers monitoring human/environmental exposure to TCC to better understand the health effects.
UR - http://www.scopus.com/inward/record.url?scp=84855312292&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855312292&partnerID=8YFLogxK
U2 - 10.1021/es202494d
DO - 10.1021/es202494d
M3 - Article
C2 - 22077920
AN - SCOPUS:84855312292
VL - 46
SP - 374
EP - 381
JO - Environmental Science & Technology
JF - Environmental Science & Technology
SN - 0013-936X
IS - 1
ER -