An immunoassay for the detection of triclosan-O-glucuronide, a primary human urinary metabolite of triclosan

Anupama Ranganathan, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Triclosan-O-glucuronide (TCSG) is one of the primary urinary metabolites of the antibacterial compound triclosan or TCS that is found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for the detection of the target TCSG in urine. Such an ELISA for TCSG could be developed as a useful tool to measure this important biomarker of human exposure to TCS. Immunogens were prepared by conjugating TCSG to thyroglobulin, via heterobifunctional cross-linkers AEDP or 3-[(2-aminoethyl)dithio] propionic acid•hydrochloride and TFCS or N-[ε-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was prepared by the direct conjugation of TCSG to bovine serum albumin. Antibodies raised in rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), and 2623 (immunogen TCSG-TFCS-Thy), and the coating antigen were screened and characterized to determine their optimal concentrations. The optimized ELISA, developed with antibody 2621, gave an IC50 value of 2.85 ng/mL, with the linear range (IC20–IC80) determined to be 2.6–24.8 ng/mL. Selectivity of the assay was assessed by measuring cross-reactivity of antibody 2621 to related congeners such as the aglycone TCS, triclosan-O-sulfate, triclocarban, a polybrominated diphenyl ether derivative, and 3-phenoxybenzyl alcohol glucuronide. There was virtually no recognition by antibody 2621 to any of these cross-reactants. [Figure not available: see fulltext.]

Original languageEnglish (US)
JournalAnalytical and Bioanalytical Chemistry
Volume407
Issue number24
DOIs
StatePublished - Jan 1 2017

Keywords

  • Biomarker
  • ELISA
  • Polyclonal antibodies
  • Triclosan-O-glucuronide
  • Urinary metabolite

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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