An immunoarray for the simultaneous determination of multiple triazine herbicides

Monika Wortberg, Sabine B. Kreissig, Geoffrey Jones, David M Rocke, Bruce D. Hammock

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

An immunochemical method for simultaneous analysis of cross-reacting analytes is presented. We demonstrate the general principle using triazine herbicides as the model system. The analysis is based on a combination of individual enzyme immunoassays (immunoarray) for triazine herbicides using antibodies with different cross-reactivity patterns towards the selected analytes. The assay signals obtained can be mathematically evaluated to estimate concentrations of each analyte out of a ternary or quaternary mixture. The mathematical model utilizes an extension of the empirical four parameter log-logistic fit. Using mono- and polyclonal antibodies it was possible to quantify the four analytes atrazine, simazine, cyanazine, and prometon in the low to sub-ppb range simultaneously.

Original languageEnglish (US)
Pages (from-to)339-352
Number of pages14
JournalAnalytica Chimica Acta
Volume304
Issue number3
DOIs
StatePublished - Apr 10 1995

Keywords

  • Immunoassay
  • Triazine herbicides

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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