Abstract
A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation often measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 131-134 |
| Number of pages | 4 |
| Journal | Archivos de Medicina Veterinaria |
| Volume | 31 |
| Issue number | 1 |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- Clostridium perfringens
- Epsilon toxin antibodies
- Fluorescence polarization assay
- Goats
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
An homogeneous fluorescence polarization assay for detection of Clostridium perfringens type D epsilon antitoxin in serum of goats. / Uzal, Francisco A; Smith, P.; Nielsen, K.; Lin, M.; Kelly, W. R.
In: Archivos de Medicina Veterinaria, Vol. 31, No. 1, 1999, p. 131-134.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An homogeneous fluorescence polarization assay for detection of Clostridium perfringens type D epsilon antitoxin in serum of goats
AU - Uzal, Francisco A
AU - Smith, P.
AU - Nielsen, K.
AU - Lin, M.
AU - Kelly, W. R.
PY - 1999
Y1 - 1999
N2 - A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation often measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later.
AB - A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation often measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later.
KW - Clostridium perfringens
KW - Epsilon toxin antibodies
KW - Fluorescence polarization assay
KW - Goats
UR - http://www.scopus.com/inward/record.url?scp=0033455366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033455366&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0033455366
VL - 31
SP - 131
EP - 134
JO - Austral journal of veterinary sciences
JF - Austral journal of veterinary sciences
SN - 0719-8000
IS - 1
ER -