Abstract
A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation often measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later.
Original language | English (US) |
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Pages (from-to) | 131-134 |
Number of pages | 4 |
Journal | Archivos de Medicina Veterinaria |
Volume | 31 |
Issue number | 1 |
State | Published - 1999 |
Externally published | Yes |
Keywords
- Clostridium perfringens
- Epsilon toxin antibodies
- Fluorescence polarization assay
- Goats
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)