An homogeneous fluorescence polarization assay for detection of Clostridium perfringens type D epsilon antitoxin in serum of goats

Francisco A Uzal, P. Smith, K. Nielsen, M. Lin, W. R. Kelly

Research output: Contribution to journalArticle

Abstract

A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation often measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalArchivos de Medicina Veterinaria
Volume31
Issue number1
StatePublished - 1999
Externally publishedYes

Keywords

  • Clostridium perfringens
  • Epsilon toxin antibodies
  • Fluorescence polarization assay
  • Goats

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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