An experimental model of allergic asthma in cats sensitized to house dust mite or Bermuda grass allergen

Carol R. Norris Reinero, Kendra C. Decile, Roy D. Berghaus, Kurt J. Williams, Christian M. Leutenegger, William F. Walby, Edward S Schelegle, Dallas M. Hyde, Laurel J Gershwin

Research output: Contribution to journalArticlepeer-review

96 Scopus citations


Background: Animal models are used to mimic human asthma, however, not all models replicate the major characteristics of the human disease. Spontaneous development of asthma with hallmark features similar to humans has been documented to occur with relative frequency in only one animal species, the cat. We hypothesized that we could develop an experimental model of feline asthma using clinically relevant aeroallergens identified from cases of naturally developing feline asthma, and characterize immunologic, physiologic, and pathologic changes over 1 year. Methods: House dust mite (HDMA) and Bermuda grass (BGA) allergen were selected by screening 10 privately owned pet cats with spontaneous asthma using a serum allergen-specific IgE ELISA. Parenteral sensitization and aerosol challenges were used to replicate the naturally developing disease in research cats. The asthmatic phenotype was characterized using intradermal skin testing, serum allergenspecific IgE ELISA, serum and bronchoalveolar lavage fluid (BALF) IgG and IgA ELISAs, airway hyperresponsiveness testing, BALF cytology, cytokine profiles using TaqMan PCR, and histopathologic evaluation. Results: Sensitization with HDMA or BGA in cats led to allergenspecific IgE production, allergen-specific serum and BALF IgG and IgA production, airway hyperreactivity, airway eosinophilia, an acute T helper 2 cytokine profile in peripheral blood mononuclear cells and BALF cells, and histologic evidence of airway remodeling. Conclusions: Using clinically relevant aeroallergens to sensitize and challenge the cat provides an additional animal model to study the immunopathophysiologic mechanisms of allergic asthma. Chronic exposure to allergen in the cat leads to a variety of immunologic, physiologic, and pathologic changes that mimic the features seen in human asthma.

Original languageEnglish (US)
Pages (from-to)117-131
Number of pages15
JournalInternational Archives of Allergy and Immunology
Issue number2
StatePublished - 2004


  • Airway hyperreactivity
  • Airway remodeling
  • Allergy
  • Animal model
  • Chronic allergen challenge
  • Feline
  • IgE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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