Abstract
Background: Animal models are used to mimic human asthma, however, not all models replicate the major characteristics of the human disease. Spontaneous development of asthma with hallmark features similar to humans has been documented to occur with relative frequency in only one animal species, the cat. We hypothesized that we could develop an experimental model of feline asthma using clinically relevant aeroallergens identified from cases of naturally developing feline asthma, and characterize immunologic, physiologic, and pathologic changes over 1 year. Methods: House dust mite (HDMA) and Bermuda grass (BGA) allergen were selected by screening 10 privately owned pet cats with spontaneous asthma using a serum allergen-specific IgE ELISA. Parenteral sensitization and aerosol challenges were used to replicate the naturally developing disease in research cats. The asthmatic phenotype was characterized using intradermal skin testing, serum allergenspecific IgE ELISA, serum and bronchoalveolar lavage fluid (BALF) IgG and IgA ELISAs, airway hyperresponsiveness testing, BALF cytology, cytokine profiles using TaqMan PCR, and histopathologic evaluation. Results: Sensitization with HDMA or BGA in cats led to allergenspecific IgE production, allergen-specific serum and BALF IgG and IgA production, airway hyperreactivity, airway eosinophilia, an acute T helper 2 cytokine profile in peripheral blood mononuclear cells and BALF cells, and histologic evidence of airway remodeling. Conclusions: Using clinically relevant aeroallergens to sensitize and challenge the cat provides an additional animal model to study the immunopathophysiologic mechanisms of allergic asthma. Chronic exposure to allergen in the cat leads to a variety of immunologic, physiologic, and pathologic changes that mimic the features seen in human asthma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 117-131 |
| Number of pages | 15 |
| Journal | International Archives of Allergy and Immunology |
| Volume | 135 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2004 |
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Keywords
- Airway hyperreactivity
- Airway remodeling
- Allergy
- Animal model
- Chronic allergen challenge
- Feline
- IgE
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
An experimental model of allergic asthma in cats sensitized to house dust mite or Bermuda grass allergen. / Norris Reinero, Carol R.; Decile, Kendra C.; Berghaus, Roy D.; Williams, Kurt J.; Leutenegger, Christian M.; Walby, William F.; Schelegle, Edward S; Hyde, Dallas M.; Gershwin, Laurel J.
In: International Archives of Allergy and Immunology, Vol. 135, No. 2, 2004, p. 117-131.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An experimental model of allergic asthma in cats sensitized to house dust mite or Bermuda grass allergen
AU - Norris Reinero, Carol R.
AU - Decile, Kendra C.
AU - Berghaus, Roy D.
AU - Williams, Kurt J.
AU - Leutenegger, Christian M.
AU - Walby, William F.
AU - Schelegle, Edward S
AU - Hyde, Dallas M.
AU - Gershwin, Laurel J
PY - 2004
Y1 - 2004
N2 - Background: Animal models are used to mimic human asthma, however, not all models replicate the major characteristics of the human disease. Spontaneous development of asthma with hallmark features similar to humans has been documented to occur with relative frequency in only one animal species, the cat. We hypothesized that we could develop an experimental model of feline asthma using clinically relevant aeroallergens identified from cases of naturally developing feline asthma, and characterize immunologic, physiologic, and pathologic changes over 1 year. Methods: House dust mite (HDMA) and Bermuda grass (BGA) allergen were selected by screening 10 privately owned pet cats with spontaneous asthma using a serum allergen-specific IgE ELISA. Parenteral sensitization and aerosol challenges were used to replicate the naturally developing disease in research cats. The asthmatic phenotype was characterized using intradermal skin testing, serum allergenspecific IgE ELISA, serum and bronchoalveolar lavage fluid (BALF) IgG and IgA ELISAs, airway hyperresponsiveness testing, BALF cytology, cytokine profiles using TaqMan PCR, and histopathologic evaluation. Results: Sensitization with HDMA or BGA in cats led to allergenspecific IgE production, allergen-specific serum and BALF IgG and IgA production, airway hyperreactivity, airway eosinophilia, an acute T helper 2 cytokine profile in peripheral blood mononuclear cells and BALF cells, and histologic evidence of airway remodeling. Conclusions: Using clinically relevant aeroallergens to sensitize and challenge the cat provides an additional animal model to study the immunopathophysiologic mechanisms of allergic asthma. Chronic exposure to allergen in the cat leads to a variety of immunologic, physiologic, and pathologic changes that mimic the features seen in human asthma.
AB - Background: Animal models are used to mimic human asthma, however, not all models replicate the major characteristics of the human disease. Spontaneous development of asthma with hallmark features similar to humans has been documented to occur with relative frequency in only one animal species, the cat. We hypothesized that we could develop an experimental model of feline asthma using clinically relevant aeroallergens identified from cases of naturally developing feline asthma, and characterize immunologic, physiologic, and pathologic changes over 1 year. Methods: House dust mite (HDMA) and Bermuda grass (BGA) allergen were selected by screening 10 privately owned pet cats with spontaneous asthma using a serum allergen-specific IgE ELISA. Parenteral sensitization and aerosol challenges were used to replicate the naturally developing disease in research cats. The asthmatic phenotype was characterized using intradermal skin testing, serum allergenspecific IgE ELISA, serum and bronchoalveolar lavage fluid (BALF) IgG and IgA ELISAs, airway hyperresponsiveness testing, BALF cytology, cytokine profiles using TaqMan PCR, and histopathologic evaluation. Results: Sensitization with HDMA or BGA in cats led to allergenspecific IgE production, allergen-specific serum and BALF IgG and IgA production, airway hyperreactivity, airway eosinophilia, an acute T helper 2 cytokine profile in peripheral blood mononuclear cells and BALF cells, and histologic evidence of airway remodeling. Conclusions: Using clinically relevant aeroallergens to sensitize and challenge the cat provides an additional animal model to study the immunopathophysiologic mechanisms of allergic asthma. Chronic exposure to allergen in the cat leads to a variety of immunologic, physiologic, and pathologic changes that mimic the features seen in human asthma.
KW - Airway hyperreactivity
KW - Airway remodeling
KW - Allergy
KW - Animal model
KW - Chronic allergen challenge
KW - Feline
KW - IgE
UR - http://www.scopus.com/inward/record.url?scp=5644227352&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=5644227352&partnerID=8YFLogxK
U2 - 10.1159/000080654
DO - 10.1159/000080654
M3 - Article
VL - 135
SP - 117
EP - 131
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 2
ER -