An ex vivo model for reperfusion of laryngotracheal grafts

Emma Barker, Paolo Macchiarini, Pamela Murison, Alan Jones, Karin Haverson, Mick Bailey, Martin Birchall

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Objective: To describe the development of an ex vivo model to facilitate the study of ischemia-reperfusiom injury in laryngotracheal grafts taken from Minnesota minipigs. Study Design: This is a descriptive study. Methods: Laryngotracheal grafts from Minnesota minipigs were harvested and placed in cold storage for 3 hours. Autologous blood was used to reperfuse the graft for up to 8 hours using the described reperfusion model. Results: By altering retrieval technique and pressures within the graft, we demonstrated healthy grafts after 8 hours of reperfusion. Conclusions: Ex vivo reperfusion offers a range of experimental advantages over in vivo reperfusion including close control of interventions, ease of outcome measurement, and reduction in animal use. This model is now ready to be used for further studies of interventions to reduce ischemia-reperfusion injury in these grafts.

Original languageEnglish (US)
Pages (from-to)699-702
Number of pages4
JournalLaryngoscope
Volume115
Issue number4
DOIs
StatePublished - Apr 2005
Externally publishedYes

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Keywords

  • Ex-vivo
  • Larynx
  • Reperfusion
  • Trachea

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Barker, E., Macchiarini, P., Murison, P., Jones, A., Haverson, K., Bailey, M., & Birchall, M. (2005). An ex vivo model for reperfusion of laryngotracheal grafts. Laryngoscope, 115(4), 699-702. https://doi.org/10.1097/01.mlg.0000161330.75600.4b