Abstract
Objective - To evaluate a combination of MNA 715 and microemulsifed cyclosporine for the prevention of renal allograft rejection in mismatched mongrel dogs. Study Design - Randomized, experimental study. Animals - Fourteen female mismatched mongrel dogs. Methods - Heterotopic renal transplantation and bilateral nephrectomy were performed in each dog. Dogs were randomly assigned to receive either MNA 715 and cyclosporine (n = 8) or cyclosporine alone (n = 6). Dogs were killed at 100 days after transplantation or when plasma creatinine exceeded 7 mg/dL. Results - In the cyclosporine and MNA 715 group: 4 dogs survived to 100 days with normal plasma creatinine concentrations; 2 dogs with intestinal intussusceptions were killed at 5 and 8 days, 1 dog with a wound infection and sepsis was killed at 14 days, and 1 dog with a serum creatinine concentration >7 mg/dL was killed at 51 days postoperatively. In the cyclosporine-alone group: 3 dogs with acute rejection were killed at 6 to 9 days and 3 dogs survived to 100 days. In dogs treated with cyclosporine and MNA 715, survival to histologically confirmed acute rejection was significantly longer (P = .044) and the degree of mononuclear cell infiltration was significantly reduced (P = .040), compared with dogs treated with cyclosporine alone. Conclusions - MNA 715 combined with cyclosporine prolonged allograft survival and reduced the severity of histologic rejection in a clinically relevant renal transplant model. Clinical Relevance - An immunosuppressive regimen consisting of MNA715 and microemulsified cyclosporine may be effective in preventing allograft rejection in canine renal transplant patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 358-366 |
| Number of pages | 9 |
| Journal | Veterinary Surgery |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2002 |
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ASJC Scopus subject areas
- veterinary(all)
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An evaluation of combined immunosuppression with MNA 715 and microemulsified cyclosporine on renal allograft rejection in mismatched mongrel dogs. / Kyles, Andrew E.; Gregory, Clare R.; Griffey, Stephen M.; Jackson, Joshua; Bernsteen, Lynda; Morris, Randall E.
In: Veterinary Surgery, Vol. 31, No. 4, 07.2002, p. 358-366.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An evaluation of combined immunosuppression with MNA 715 and microemulsified cyclosporine on renal allograft rejection in mismatched mongrel dogs
AU - Kyles, Andrew E.
AU - Gregory, Clare R.
AU - Griffey, Stephen M.
AU - Jackson, Joshua
AU - Bernsteen, Lynda
AU - Morris, Randall E.
PY - 2002/7
Y1 - 2002/7
N2 - Objective - To evaluate a combination of MNA 715 and microemulsifed cyclosporine for the prevention of renal allograft rejection in mismatched mongrel dogs. Study Design - Randomized, experimental study. Animals - Fourteen female mismatched mongrel dogs. Methods - Heterotopic renal transplantation and bilateral nephrectomy were performed in each dog. Dogs were randomly assigned to receive either MNA 715 and cyclosporine (n = 8) or cyclosporine alone (n = 6). Dogs were killed at 100 days after transplantation or when plasma creatinine exceeded 7 mg/dL. Results - In the cyclosporine and MNA 715 group: 4 dogs survived to 100 days with normal plasma creatinine concentrations; 2 dogs with intestinal intussusceptions were killed at 5 and 8 days, 1 dog with a wound infection and sepsis was killed at 14 days, and 1 dog with a serum creatinine concentration >7 mg/dL was killed at 51 days postoperatively. In the cyclosporine-alone group: 3 dogs with acute rejection were killed at 6 to 9 days and 3 dogs survived to 100 days. In dogs treated with cyclosporine and MNA 715, survival to histologically confirmed acute rejection was significantly longer (P = .044) and the degree of mononuclear cell infiltration was significantly reduced (P = .040), compared with dogs treated with cyclosporine alone. Conclusions - MNA 715 combined with cyclosporine prolonged allograft survival and reduced the severity of histologic rejection in a clinically relevant renal transplant model. Clinical Relevance - An immunosuppressive regimen consisting of MNA715 and microemulsified cyclosporine may be effective in preventing allograft rejection in canine renal transplant patients.
AB - Objective - To evaluate a combination of MNA 715 and microemulsifed cyclosporine for the prevention of renal allograft rejection in mismatched mongrel dogs. Study Design - Randomized, experimental study. Animals - Fourteen female mismatched mongrel dogs. Methods - Heterotopic renal transplantation and bilateral nephrectomy were performed in each dog. Dogs were randomly assigned to receive either MNA 715 and cyclosporine (n = 8) or cyclosporine alone (n = 6). Dogs were killed at 100 days after transplantation or when plasma creatinine exceeded 7 mg/dL. Results - In the cyclosporine and MNA 715 group: 4 dogs survived to 100 days with normal plasma creatinine concentrations; 2 dogs with intestinal intussusceptions were killed at 5 and 8 days, 1 dog with a wound infection and sepsis was killed at 14 days, and 1 dog with a serum creatinine concentration >7 mg/dL was killed at 51 days postoperatively. In the cyclosporine-alone group: 3 dogs with acute rejection were killed at 6 to 9 days and 3 dogs survived to 100 days. In dogs treated with cyclosporine and MNA 715, survival to histologically confirmed acute rejection was significantly longer (P = .044) and the degree of mononuclear cell infiltration was significantly reduced (P = .040), compared with dogs treated with cyclosporine alone. Conclusions - MNA 715 combined with cyclosporine prolonged allograft survival and reduced the severity of histologic rejection in a clinically relevant renal transplant model. Clinical Relevance - An immunosuppressive regimen consisting of MNA715 and microemulsified cyclosporine may be effective in preventing allograft rejection in canine renal transplant patients.
UR - http://www.scopus.com/inward/record.url?scp=0036651222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036651222&partnerID=8YFLogxK
U2 - 10.1053/jvet.2002.33615
DO - 10.1053/jvet.2002.33615
M3 - Article
C2 - 12094350
AN - SCOPUS:0036651222
VL - 31
SP - 358
EP - 366
JO - Veterinary Surgery
JF - Veterinary Surgery
SN - 0161-3499
IS - 4
ER -