An ER-mitochondria tethering complex revealed by a synthetic biology screen

Benoît Kornmann, Erin Currie, Sean R. Collins, Maya Schuldiner, Jodi Nunnari, Jonathan S. Weissman, Peter Walter

Research output: Contribution to journalArticle

721 Scopus citations

Abstract

Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalScience
Volume325
Issue number5939
DOIs
StatePublished - Jul 24 2009

ASJC Scopus subject areas

  • General

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    Kornmann, B., Currie, E., Collins, S. R., Schuldiner, M., Nunnari, J., Weissman, J. S., & Walter, P. (2009). An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science, 325(5939), 477-481. https://doi.org/10.1126/science.1175088