Abstract
Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 477-481 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 325 |
| Issue number | 5939 |
| DOIs | |
| State | Published - Jul 24 2009 |
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An ER-mitochondria tethering complex revealed by a synthetic biology screen. / Kornmann, Benoît; Currie, Erin; Collins, Sean; Schuldiner, Maya; Nunnari, Jodi; Weissman, Jonathan S.; Walter, Peter.
In: Science, Vol. 325, No. 5939, 24.07.2009, p. 477-481.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An ER-mitochondria tethering complex revealed by a synthetic biology screen
AU - Kornmann, Benoît
AU - Currie, Erin
AU - Collins, Sean
AU - Schuldiner, Maya
AU - Nunnari, Jodi
AU - Weissman, Jonathan S.
AU - Walter, Peter
PY - 2009/7/24
Y1 - 2009/7/24
N2 - Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.
AB - Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.
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U2 - 10.1126/science.1175088
DO - 10.1126/science.1175088
M3 - Article
VL - 325
SP - 477
EP - 481
JO - Science
JF - Science
SN - 0036-8075
IS - 5939
ER -