An ER-mitochondria tethering complex revealed by a synthetic biology screen

Benoît Kornmann, Erin Currie, Sean R. Collins, Maya Schuldiner, Jodi Nunnari, Jonathan S. Weissman, Peter Walter

Research output: Contribution to journalArticle

684 Citations (Scopus)

Abstract

Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalScience
Volume325
Issue number5939
DOIs
StatePublished - Jul 24 2009

Fingerprint

Synthetic Biology
Endoplasmic Reticulum
Mitochondria
Phospholipids
Organelles
Calcium Signaling
Chromosome Mapping
Eukaryotic Cells
Proteins
Calcium
Genes

ASJC Scopus subject areas

  • General

Cite this

Kornmann, B., Currie, E., Collins, S. R., Schuldiner, M., Nunnari, J., Weissman, J. S., & Walter, P. (2009). An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science, 325(5939), 477-481. https://doi.org/10.1126/science.1175088

An ER-mitochondria tethering complex revealed by a synthetic biology screen. / Kornmann, Benoît; Currie, Erin; Collins, Sean R.; Schuldiner, Maya; Nunnari, Jodi; Weissman, Jonathan S.; Walter, Peter.

In: Science, Vol. 325, No. 5939, 24.07.2009, p. 477-481.

Research output: Contribution to journalArticle

Kornmann, B, Currie, E, Collins, SR, Schuldiner, M, Nunnari, J, Weissman, JS & Walter, P 2009, 'An ER-mitochondria tethering complex revealed by a synthetic biology screen', Science, vol. 325, no. 5939, pp. 477-481. https://doi.org/10.1126/science.1175088
Kornmann B, Currie E, Collins SR, Schuldiner M, Nunnari J, Weissman JS et al. An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science. 2009 Jul 24;325(5939):477-481. https://doi.org/10.1126/science.1175088
Kornmann, Benoît ; Currie, Erin ; Collins, Sean R. ; Schuldiner, Maya ; Nunnari, Jodi ; Weissman, Jonathan S. ; Walter, Peter. / An ER-mitochondria tethering complex revealed by a synthetic biology screen. In: Science. 2009 ; Vol. 325, No. 5939. pp. 477-481.
@article{d00dc0da801e4d9095c9f0237d106a85,
title = "An ER-mitochondria tethering complex revealed by a synthetic biology screen",
abstract = "Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.",
author = "Beno{\^i}t Kornmann and Erin Currie and Collins, {Sean R.} and Maya Schuldiner and Jodi Nunnari and Weissman, {Jonathan S.} and Peter Walter",
year = "2009",
month = "7",
day = "24",
doi = "10.1126/science.1175088",
language = "English (US)",
volume = "325",
pages = "477--481",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5939",

}

TY - JOUR

T1 - An ER-mitochondria tethering complex revealed by a synthetic biology screen

AU - Kornmann, Benoît

AU - Currie, Erin

AU - Collins, Sean R.

AU - Schuldiner, Maya

AU - Nunnari, Jodi

AU - Weissman, Jonathan S.

AU - Walter, Peter

PY - 2009/7/24

Y1 - 2009/7/24

N2 - Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.

AB - Communication between organelles is an important feature of all eukaryotic cells. To uncover components involved in mitochondria/endoplasmic reticulum (ER) junctions, we screened for mutants that could be complemented by a synthetic protein designed to artificially tether the two organelles. We identified the Mmm1/Mdm10/Mdm12/Mdm34 complex as a molecular tether between ER and mitochondria. The tethering complex was composed of proteins resident of both ER and mitochondria. With the use of genome-wide mapping of genetic interactions, we showed that the components of the tethering complex were functionally connected to phospholipid biosynthesis and calcium-signaling genes. In mutant cells, phospholipid biosynthesis was impaired. The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange.

UR - http://www.scopus.com/inward/record.url?scp=67749122635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67749122635&partnerID=8YFLogxK

U2 - 10.1126/science.1175088

DO - 10.1126/science.1175088

M3 - Article

C2 - 19556461

AN - SCOPUS:67749122635

VL - 325

SP - 477

EP - 481

JO - Science

JF - Science

SN - 0036-8075

IS - 5939

ER -