Abstract
A 96-microwell enzyme-linked immunosorbent assay (ELISA) method was evaluated to determine PCDDs/PCDFs in sediment and soil samples from an EPA Superfund site. Samples were prepared and analyzed by both the ELISA and a gas chromatography/high resolution mass spectrometry (GC/HRMS) method. Comparable method precision, accuracy, and detection level (8 ng kg-1) were achieved by the ELISA method with respect to GC/HRMS. However, the extraction and cleanup method developed for the ELISA requires refinement for the soil type that yielded a waxy residue after sample processing. Four types of statistical analyses (Pearson correlation coefficient, paired t-test, nonparametric tests, and McNemar's test of association) were performed to determine whether the two methods produced statistically different results. The log-transformed ELISA-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin values and log-transformed GC/HRMS-derived TEQ values were significantly correlated (r = 0.79) at the 0.05 level. The median difference in values between ELISA and GC/HRMS was not significant at the 0.05 level. Low false negative and false positive rates (<10%) were observed for the ELISA when compared to the GC/HRMS at 1000 ng TEQ kg-1. The findings suggest that immunochemical technology could be a complementary monitoring tool for determining concentrations at the 1000 ng TEQ kg-1 action level for contaminated sediment and soil. The ELISA could also be used in an analytical triage approach to screen and rank samples prior to instrumental analysis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-103 |
| Number of pages | 9 |
| Journal | Chemosphere |
| Volume | 72 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 2008 |
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Keywords
- 2,3,7,8-Tetrachlorodibenzo-p-dioxin
- GC/HRMS
- Immunoassay
- PCDD
- PCDF
- TCDD
ASJC Scopus subject areas
- Environmental Chemistry
- Environmental Science(all)
Cite this
An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples. / Emon, Jeanette M Van; Chuang, Jane C.; Lordo, Robert A.; Schrock, Mary E.; Nichkova, Mikaela; Gee, Shirley J.; Hammock, Bruce D.
In: Chemosphere, Vol. 72, No. 1, 05.2008, p. 95-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples
AU - Emon, Jeanette M Van
AU - Chuang, Jane C.
AU - Lordo, Robert A.
AU - Schrock, Mary E.
AU - Nichkova, Mikaela
AU - Gee, Shirley J.
AU - Hammock, Bruce D.
PY - 2008/5
Y1 - 2008/5
N2 - A 96-microwell enzyme-linked immunosorbent assay (ELISA) method was evaluated to determine PCDDs/PCDFs in sediment and soil samples from an EPA Superfund site. Samples were prepared and analyzed by both the ELISA and a gas chromatography/high resolution mass spectrometry (GC/HRMS) method. Comparable method precision, accuracy, and detection level (8 ng kg-1) were achieved by the ELISA method with respect to GC/HRMS. However, the extraction and cleanup method developed for the ELISA requires refinement for the soil type that yielded a waxy residue after sample processing. Four types of statistical analyses (Pearson correlation coefficient, paired t-test, nonparametric tests, and McNemar's test of association) were performed to determine whether the two methods produced statistically different results. The log-transformed ELISA-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin values and log-transformed GC/HRMS-derived TEQ values were significantly correlated (r = 0.79) at the 0.05 level. The median difference in values between ELISA and GC/HRMS was not significant at the 0.05 level. Low false negative and false positive rates (<10%) were observed for the ELISA when compared to the GC/HRMS at 1000 ng TEQ kg-1. The findings suggest that immunochemical technology could be a complementary monitoring tool for determining concentrations at the 1000 ng TEQ kg-1 action level for contaminated sediment and soil. The ELISA could also be used in an analytical triage approach to screen and rank samples prior to instrumental analysis.
AB - A 96-microwell enzyme-linked immunosorbent assay (ELISA) method was evaluated to determine PCDDs/PCDFs in sediment and soil samples from an EPA Superfund site. Samples were prepared and analyzed by both the ELISA and a gas chromatography/high resolution mass spectrometry (GC/HRMS) method. Comparable method precision, accuracy, and detection level (8 ng kg-1) were achieved by the ELISA method with respect to GC/HRMS. However, the extraction and cleanup method developed for the ELISA requires refinement for the soil type that yielded a waxy residue after sample processing. Four types of statistical analyses (Pearson correlation coefficient, paired t-test, nonparametric tests, and McNemar's test of association) were performed to determine whether the two methods produced statistically different results. The log-transformed ELISA-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin values and log-transformed GC/HRMS-derived TEQ values were significantly correlated (r = 0.79) at the 0.05 level. The median difference in values between ELISA and GC/HRMS was not significant at the 0.05 level. Low false negative and false positive rates (<10%) were observed for the ELISA when compared to the GC/HRMS at 1000 ng TEQ kg-1. The findings suggest that immunochemical technology could be a complementary monitoring tool for determining concentrations at the 1000 ng TEQ kg-1 action level for contaminated sediment and soil. The ELISA could also be used in an analytical triage approach to screen and rank samples prior to instrumental analysis.
KW - 2,3,7,8-Tetrachlorodibenzo-p-dioxin
KW - GC/HRMS
KW - Immunoassay
KW - PCDD
KW - PCDF
KW - TCDD
UR - http://www.scopus.com/inward/record.url?scp=42749084251&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42749084251&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2008.01.012
DO - 10.1016/j.chemosphere.2008.01.012
M3 - Article
C2 - 18313102
AN - SCOPUS:42749084251
VL - 72
SP - 95
EP - 103
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 1
ER -