An enzyme-linked immunosorbent assay for the detection of esfenvalerate metabolites in human urine

Guomin Shan, Ingrid Wengatz, Donald W. Stoutamire, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The pyrethroids are one of the most heavily used insecticide classes in the world. Sensitive and rapid analytical techniques are needed for assessments of human exposure to these compounds. Highly sensitive and selective ELISAs for glycine conjugates of esfenvalerate key metabolites phenoxybenzoic acid (PBA) and s-fenvalerate acid (sFA) were developed. Rabbits were immunized with either N-(3-phenoxybenzoyl)-4-amino-L- phenylalanine-fetuin or N-[(S)4-chloro-2-(methylethyl)benzeneacetyl]-4-amino- L-phenylalanine-fetuin, and all sera were screened against numerous coating antigens. The antibodies with the least interference and best sensitivity were optimized and characterized. The I50S for sFA-glycine and PBA-glycine in buffer were found to be 0.40 ± 0.12 μg/L (1.47 ± 0.44 nmol/L) and 0.42 ± 0.18 μ/L (1.56 ± 0.67 nmol/L), respectively. Both assays exhibited high selectivity. Little or no cross reactivity to the parent compound and other metabolites was measured. The matrix effects of urine were investigated. Solid-phase extraction (SPE) strategies were used in an attempt to decrease the matrix effects and increase the sensitivity of the overall method. The limit of quantitation (LOQ) for both sFA-glycine and PBA-glycine in urine with SPE is 1.0 μ/L (3.70 nmol/L). These assays could be used as markers of exposure for monitoring biological samples.

Original languageEnglish (US)
Pages (from-to)1033-1041
Number of pages9
JournalChemical Research in Toxicology
Volume12
Issue number11
DOIs
StatePublished - Nov 1999

Fingerprint

fenvalerate
Immunosorbents
Metabolites
Assays
Glycine
Enzyme-Linked Immunosorbent Assay
Urine
Acids
Enzymes
Fetuins
Solid Phase Extraction
Phenylalanine
Pyrethrins
Environmental Monitoring
Insecticides
Buffers
Rabbits

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

An enzyme-linked immunosorbent assay for the detection of esfenvalerate metabolites in human urine. / Shan, Guomin; Wengatz, Ingrid; Stoutamire, Donald W.; Gee, Shirley J.; Hammock, Bruce D.

In: Chemical Research in Toxicology, Vol. 12, No. 11, 11.1999, p. 1033-1041.

Research output: Contribution to journalArticle

Shan, Guomin ; Wengatz, Ingrid ; Stoutamire, Donald W. ; Gee, Shirley J. ; Hammock, Bruce D. / An enzyme-linked immunosorbent assay for the detection of esfenvalerate metabolites in human urine. In: Chemical Research in Toxicology. 1999 ; Vol. 12, No. 11. pp. 1033-1041.
@article{8d6f35c8c1e6409998203d2cbd2b89a6,
title = "An enzyme-linked immunosorbent assay for the detection of esfenvalerate metabolites in human urine",
abstract = "The pyrethroids are one of the most heavily used insecticide classes in the world. Sensitive and rapid analytical techniques are needed for assessments of human exposure to these compounds. Highly sensitive and selective ELISAs for glycine conjugates of esfenvalerate key metabolites phenoxybenzoic acid (PBA) and s-fenvalerate acid (sFA) were developed. Rabbits were immunized with either N-(3-phenoxybenzoyl)-4-amino-L- phenylalanine-fetuin or N-[(S)4-chloro-2-(methylethyl)benzeneacetyl]-4-amino- L-phenylalanine-fetuin, and all sera were screened against numerous coating antigens. The antibodies with the least interference and best sensitivity were optimized and characterized. The I50S for sFA-glycine and PBA-glycine in buffer were found to be 0.40 ± 0.12 μg/L (1.47 ± 0.44 nmol/L) and 0.42 ± 0.18 μ/L (1.56 ± 0.67 nmol/L), respectively. Both assays exhibited high selectivity. Little or no cross reactivity to the parent compound and other metabolites was measured. The matrix effects of urine were investigated. Solid-phase extraction (SPE) strategies were used in an attempt to decrease the matrix effects and increase the sensitivity of the overall method. The limit of quantitation (LOQ) for both sFA-glycine and PBA-glycine in urine with SPE is 1.0 μ/L (3.70 nmol/L). These assays could be used as markers of exposure for monitoring biological samples.",
author = "Guomin Shan and Ingrid Wengatz and Stoutamire, {Donald W.} and Gee, {Shirley J.} and Hammock, {Bruce D.}",
year = "1999",
month = "11",
doi = "10.1021/tx990091h",
language = "English (US)",
volume = "12",
pages = "1033--1041",
journal = "Chemical Research in Toxicology",
issn = "0893-228X",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - An enzyme-linked immunosorbent assay for the detection of esfenvalerate metabolites in human urine

AU - Shan, Guomin

AU - Wengatz, Ingrid

AU - Stoutamire, Donald W.

AU - Gee, Shirley J.

AU - Hammock, Bruce D.

PY - 1999/11

Y1 - 1999/11

N2 - The pyrethroids are one of the most heavily used insecticide classes in the world. Sensitive and rapid analytical techniques are needed for assessments of human exposure to these compounds. Highly sensitive and selective ELISAs for glycine conjugates of esfenvalerate key metabolites phenoxybenzoic acid (PBA) and s-fenvalerate acid (sFA) were developed. Rabbits were immunized with either N-(3-phenoxybenzoyl)-4-amino-L- phenylalanine-fetuin or N-[(S)4-chloro-2-(methylethyl)benzeneacetyl]-4-amino- L-phenylalanine-fetuin, and all sera were screened against numerous coating antigens. The antibodies with the least interference and best sensitivity were optimized and characterized. The I50S for sFA-glycine and PBA-glycine in buffer were found to be 0.40 ± 0.12 μg/L (1.47 ± 0.44 nmol/L) and 0.42 ± 0.18 μ/L (1.56 ± 0.67 nmol/L), respectively. Both assays exhibited high selectivity. Little or no cross reactivity to the parent compound and other metabolites was measured. The matrix effects of urine were investigated. Solid-phase extraction (SPE) strategies were used in an attempt to decrease the matrix effects and increase the sensitivity of the overall method. The limit of quantitation (LOQ) for both sFA-glycine and PBA-glycine in urine with SPE is 1.0 μ/L (3.70 nmol/L). These assays could be used as markers of exposure for monitoring biological samples.

AB - The pyrethroids are one of the most heavily used insecticide classes in the world. Sensitive and rapid analytical techniques are needed for assessments of human exposure to these compounds. Highly sensitive and selective ELISAs for glycine conjugates of esfenvalerate key metabolites phenoxybenzoic acid (PBA) and s-fenvalerate acid (sFA) were developed. Rabbits were immunized with either N-(3-phenoxybenzoyl)-4-amino-L- phenylalanine-fetuin or N-[(S)4-chloro-2-(methylethyl)benzeneacetyl]-4-amino- L-phenylalanine-fetuin, and all sera were screened against numerous coating antigens. The antibodies with the least interference and best sensitivity were optimized and characterized. The I50S for sFA-glycine and PBA-glycine in buffer were found to be 0.40 ± 0.12 μg/L (1.47 ± 0.44 nmol/L) and 0.42 ± 0.18 μ/L (1.56 ± 0.67 nmol/L), respectively. Both assays exhibited high selectivity. Little or no cross reactivity to the parent compound and other metabolites was measured. The matrix effects of urine were investigated. Solid-phase extraction (SPE) strategies were used in an attempt to decrease the matrix effects and increase the sensitivity of the overall method. The limit of quantitation (LOQ) for both sFA-glycine and PBA-glycine in urine with SPE is 1.0 μ/L (3.70 nmol/L). These assays could be used as markers of exposure for monitoring biological samples.

UR - http://www.scopus.com/inward/record.url?scp=0032702365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032702365&partnerID=8YFLogxK

U2 - 10.1021/tx990091h

DO - 10.1021/tx990091h

M3 - Article

C2 - 10563828

AN - SCOPUS:0032702365

VL - 12

SP - 1033

EP - 1041

JO - Chemical Research in Toxicology

JF - Chemical Research in Toxicology

SN - 0893-228X

IS - 11

ER -