An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to channel catfish virus (CCV) in channel catfish

Sara A. Crawford, Ian Gardner, Ronald Hedrick

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against channel catfish virus (CCV) in the serum of channel catfish Ictalurus punctatus. The ELISA was used to test sera from 20 waterborne CCV-exposed juvenile channel catfish and 10 unexposed channel catfish for antibodies to CCV. Each serum was tested at a single dilution (1:10, volume:volume), and positive–negative cutoff values were established as the mean of negative sera plus either 2, 3, or 4 standard deviations. The resulting ELISA was highly reproducible and accurate with sensitivities and specificities of 100% and 90%, 95% and 90%, and 95% and 100%, respectively. Most fish seroconverted between 22 and 28 d after first exposure to CCV. This immunoassay is capable of handling large numbers of samples reliably and rapidly for detection and assessing prevalence of CCV exposure in channel catfish.

Original languageEnglish (US)
Pages (from-to)148-153
Number of pages6
JournalJournal of Aquatic Animal Health
Volume11
Issue number2
DOIs
StatePublished - Jan 1 1999

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Ictalurid herpesvirus 1
Ictalurus punctatus
antibody detection
antibody
virus
enzyme-linked immunosorbent assay
assay
enzyme
serum
immunoassays
detection
immunoassay
antibodies
fish
dilution

ASJC Scopus subject areas

  • Aquatic Science

Cite this

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abstract = "An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies against channel catfish virus (CCV) in the serum of channel catfish Ictalurus punctatus. The ELISA was used to test sera from 20 waterborne CCV-exposed juvenile channel catfish and 10 unexposed channel catfish for antibodies to CCV. Each serum was tested at a single dilution (1:10, volume:volume), and positive–negative cutoff values were established as the mean of negative sera plus either 2, 3, or 4 standard deviations. The resulting ELISA was highly reproducible and accurate with sensitivities and specificities of 100{\%} and 90{\%}, 95{\%} and 90{\%}, and 95{\%} and 100{\%}, respectively. Most fish seroconverted between 22 and 28 d after first exposure to CCV. This immunoassay is capable of handling large numbers of samples reliably and rapidly for detection and assessing prevalence of CCV exposure in channel catfish.",
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