An avian bioassay for environmental estrogens: The growth response of zebra finch (Taeniopygia guttata) chick oviduct to oral estrogens

James R. Millam, Christina B. Craig-Veit, Marianne E. Batchelder, Mark R. Viant, Tina M. Herbeck, Leslie Woods

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The rat uterotrophic assay is a recommended tier 1 screening assay for environmental estrogens, but no comparable assay exists for altricial birds. We orally dosed zebra finch chicks daily during their linear growth phase (days 5-11) with estradiol benzoate (EB), genistein, methoxychlor, or octylphenol, all dissolved or suspended in canola oil, or canola oil alone, as a vehicle control. On day 12, oviducts were removed, weighed and examined histologically. All doses of EB (0.1-1,000 nmol/g body wt), genistein at 100 nmol/g, and methoxychlor and octylphenol at 1,000 nmol/g, markedly increased oviduct weight, with the highest dose of EB inducing a 60-fold increase over controls. Oviducts were differentiated in a dose-depedent manner to the point of having tubular glands and a pseudostratified, ciliated epithelium at the higher doses of EB. Our earlier results show that EB at 100 and 1,000 nmol/g impairs reproductive performance of zebra finches. Thus, the zebra finch oviduct bioassay measures estrogenicity over a wide dose range and, for EB exposure, can predict impairment in adult reproductive performance. The responsiveness of chick oviducts to estrogen stimulation may serve as a useful marker of estrogen exposure in wild populations of songbirds.

Original languageEnglish (US)
Pages (from-to)2663-2668
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume21
Issue number12
StatePublished - Dec 1 2002

Keywords

  • Endocrine disruptor
  • Estrogen bioassay
  • Oviduct
  • Phytoestrogen
  • Taeniopygia guttata

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

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