Abstract
The rat uterotrophic assay is a recommended tier 1 screening assay for environmental estrogens, but no comparable assay exists for altricial birds. We orally dosed zebra finch chicks daily during their linear growth phase (days 5-11) with estradiol benzoate (EB), genistein, methoxychlor, or octylphenol, all dissolved or suspended in canola oil, or canola oil alone, as a vehicle control. On day 12, oviducts were removed, weighed and examined histologically. All doses of EB (0.1-1,000 nmol/g body wt), genistein at 100 nmol/g, and methoxychlor and octylphenol at 1,000 nmol/g, markedly increased oviduct weight, with the highest dose of EB inducing a 60-fold increase over controls. Oviducts were differentiated in a dose-depedent manner to the point of having tubular glands and a pseudostratified, ciliated epithelium at the higher doses of EB. Our earlier results show that EB at 100 and 1,000 nmol/g impairs reproductive performance of zebra finches. Thus, the zebra finch oviduct bioassay measures estrogenicity over a wide dose range and, for EB exposure, can predict impairment in adult reproductive performance. The responsiveness of chick oviducts to estrogen stimulation may serve as a useful marker of estrogen exposure in wild populations of songbirds.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2663-2668 |
| Number of pages | 6 |
| Journal | Environmental Toxicology and Chemistry |
| Volume | 21 |
| Issue number | 12 |
| State | Published - Dec 1 2002 |
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Keywords
- Endocrine disruptor
- Estrogen bioassay
- Oviduct
- Phytoestrogen
- Taeniopygia guttata
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
An avian bioassay for environmental estrogens : The growth response of zebra finch (Taeniopygia guttata) chick oviduct to oral estrogens. / Millam, James R.; Craig-Veit, Christina B.; Batchelder, Marianne E.; Viant, Mark R.; Herbeck, Tina M.; Woods, Leslie.
In: Environmental Toxicology and Chemistry, Vol. 21, No. 12, 01.12.2002, p. 2663-2668.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An avian bioassay for environmental estrogens
T2 - The growth response of zebra finch (Taeniopygia guttata) chick oviduct to oral estrogens
AU - Millam, James R.
AU - Craig-Veit, Christina B.
AU - Batchelder, Marianne E.
AU - Viant, Mark R.
AU - Herbeck, Tina M.
AU - Woods, Leslie
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The rat uterotrophic assay is a recommended tier 1 screening assay for environmental estrogens, but no comparable assay exists for altricial birds. We orally dosed zebra finch chicks daily during their linear growth phase (days 5-11) with estradiol benzoate (EB), genistein, methoxychlor, or octylphenol, all dissolved or suspended in canola oil, or canola oil alone, as a vehicle control. On day 12, oviducts were removed, weighed and examined histologically. All doses of EB (0.1-1,000 nmol/g body wt), genistein at 100 nmol/g, and methoxychlor and octylphenol at 1,000 nmol/g, markedly increased oviduct weight, with the highest dose of EB inducing a 60-fold increase over controls. Oviducts were differentiated in a dose-depedent manner to the point of having tubular glands and a pseudostratified, ciliated epithelium at the higher doses of EB. Our earlier results show that EB at 100 and 1,000 nmol/g impairs reproductive performance of zebra finches. Thus, the zebra finch oviduct bioassay measures estrogenicity over a wide dose range and, for EB exposure, can predict impairment in adult reproductive performance. The responsiveness of chick oviducts to estrogen stimulation may serve as a useful marker of estrogen exposure in wild populations of songbirds.
AB - The rat uterotrophic assay is a recommended tier 1 screening assay for environmental estrogens, but no comparable assay exists for altricial birds. We orally dosed zebra finch chicks daily during their linear growth phase (days 5-11) with estradiol benzoate (EB), genistein, methoxychlor, or octylphenol, all dissolved or suspended in canola oil, or canola oil alone, as a vehicle control. On day 12, oviducts were removed, weighed and examined histologically. All doses of EB (0.1-1,000 nmol/g body wt), genistein at 100 nmol/g, and methoxychlor and octylphenol at 1,000 nmol/g, markedly increased oviduct weight, with the highest dose of EB inducing a 60-fold increase over controls. Oviducts were differentiated in a dose-depedent manner to the point of having tubular glands and a pseudostratified, ciliated epithelium at the higher doses of EB. Our earlier results show that EB at 100 and 1,000 nmol/g impairs reproductive performance of zebra finches. Thus, the zebra finch oviduct bioassay measures estrogenicity over a wide dose range and, for EB exposure, can predict impairment in adult reproductive performance. The responsiveness of chick oviducts to estrogen stimulation may serve as a useful marker of estrogen exposure in wild populations of songbirds.
KW - Endocrine disruptor
KW - Estrogen bioassay
KW - Oviduct
KW - Phytoestrogen
KW - Taeniopygia guttata
UR - http://www.scopus.com/inward/record.url?scp=0036889132&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036889132&partnerID=8YFLogxK
M3 - Article
C2 - 12463562
AN - SCOPUS:0036889132
VL - 21
SP - 2663
EP - 2668
JO - Environmental Toxicology and Chemistry
JF - Environmental Toxicology and Chemistry
SN - 0730-7268
IS - 12
ER -