An autocrine motility factor secreted by the Dunning R-3327 rat prostatic adenocarcinoma cell subtype AT2.1

Christopher P Evans, D. S. Walsh, E. C. Kohn

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Abstract

Tumor cell locomotion is an integral part of the metastatic process. We present a new autocrine motility factor (AMF) derived from the serum-free conditioned medium of the Dunning R-3327 rat prostate adenocarcinoma AT2.1 tumor cell subline AT2.1-AMF, prepared by concentration of components ≤ 30 kDa in size and washed free of low-molecular weight growth factors, stimulated motility of AT2.1 cells in modified Boyden chamber migration assays. This stimulated migration was dose-dependent, and by checkerboard analysis was both chemotactic and chemokinetic. AT2.1-AMF activity was labile to heat, acid, base, reduction, oxidation, and proteases. Lyophilization and treatment with 6M urea caused a mild decrease (< 20%) in migration-stimulating capability. Tumor-cell specificity was demonstrated for AMF of AT2.1 and AT3.1 Dunning sublines, and the A2058 human melanoma cell lines. AT2.1 cell migration to AT2.1-AMF was inhibited by 2 hr pre-treatment with cholera toxin (0.1 μg/ml) or forskolin (100 μM), but not altered by 2 hr pre-treatment with pertussis toxin (1.0 μg/ml). This indicates that guanine nucleotide binding protein-mediated regulation of cAMP is involved in modulating the AT2.1 cell response to its AMF. The AT2.1-AMF belongs to a related family of tumor autocrine motility factors and represents a new model for understanding the role of tumor-cell migration in the metastatic process of human prostate cancer.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalInternational Journal of Cancer
Volume49
Issue number1
StatePublished - 1991

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Glucose-6-Phosphate Isomerase
Adenocarcinoma
Cell Movement
Neoplasms
Guanine Nucleotides
Freeze Drying
Cholera Toxin
Serum-Free Culture Media
Pertussis Toxin
Colforsin
Conditioned Culture Medium
Urea
Prostate
Melanoma
Prostatic Neoplasms
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Peptide Hydrolases
Therapeutics
Hot Temperature

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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An autocrine motility factor secreted by the Dunning R-3327 rat prostatic adenocarcinoma cell subtype AT2.1. / Evans, Christopher P; Walsh, D. S.; Kohn, E. C.

In: International Journal of Cancer, Vol. 49, No. 1, 1991, p. 109-113.

Research output: Contribution to journalArticle

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