Abstract
We demonstrate the construction of an immunoarray to categorize, identify and quantitate different triazine herbicides or their environmental metabolites in water. In a first step cluster analysis is used to examine the performance of a small subset of antibodies out of a larger library, and hence to select a small array that is capable of categorizing the triazine herbicides into different groups. At the 1 ppb level it is possible to categorize an analyte as a chloro-s-triazine, a hydroxy metabolite, or as a methoxy/methylthio substituted triazine when using only 2-4 antibodies. At higher concentrations even the identity of the triazine can be determined with the same number of antibodies. A selected combination of antibodies is then used to identify and quantify unknowns by comparing their immunoassay responses to an array of calibration curves and using the maximum likelihood criterion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 291-303 |
| Number of pages | 13 |
| Journal | Analytica Chimica Acta |
| Volume | 319 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 9 1996 |
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Keywords
- Environmental analysis
- Herbicides
- Immunoarray construction
- Triazine herbicides
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes. / Wortberg, M.; Jones, G.; Kreissig, S. B.; Rocke, David M; Gee, S. J.; Hammock, B. D.
In: Analytica Chimica Acta, Vol. 319, No. 3, 09.02.1996, p. 291-303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes
AU - Wortberg, M.
AU - Jones, G.
AU - Kreissig, S. B.
AU - Rocke, David M
AU - Gee, S. J.
AU - Hammock, B. D.
PY - 1996/2/9
Y1 - 1996/2/9
N2 - We demonstrate the construction of an immunoarray to categorize, identify and quantitate different triazine herbicides or their environmental metabolites in water. In a first step cluster analysis is used to examine the performance of a small subset of antibodies out of a larger library, and hence to select a small array that is capable of categorizing the triazine herbicides into different groups. At the 1 ppb level it is possible to categorize an analyte as a chloro-s-triazine, a hydroxy metabolite, or as a methoxy/methylthio substituted triazine when using only 2-4 antibodies. At higher concentrations even the identity of the triazine can be determined with the same number of antibodies. A selected combination of antibodies is then used to identify and quantify unknowns by comparing their immunoassay responses to an array of calibration curves and using the maximum likelihood criterion.
AB - We demonstrate the construction of an immunoarray to categorize, identify and quantitate different triazine herbicides or their environmental metabolites in water. In a first step cluster analysis is used to examine the performance of a small subset of antibodies out of a larger library, and hence to select a small array that is capable of categorizing the triazine herbicides into different groups. At the 1 ppb level it is possible to categorize an analyte as a chloro-s-triazine, a hydroxy metabolite, or as a methoxy/methylthio substituted triazine when using only 2-4 antibodies. At higher concentrations even the identity of the triazine can be determined with the same number of antibodies. A selected combination of antibodies is then used to identify and quantify unknowns by comparing their immunoassay responses to an array of calibration curves and using the maximum likelihood criterion.
KW - Environmental analysis
KW - Herbicides
KW - Immunoarray construction
KW - Triazine herbicides
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UR - http://www.scopus.com/inward/citedby.url?scp=0030062149&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(95)00500-5
DO - 10.1016/0003-2670(95)00500-5
M3 - Article
AN - SCOPUS:0030062149
VL - 319
SP - 291
EP - 303
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 3
ER -