An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes

M. Wortberg, G. Jones, S. B. Kreissig, David M Rocke, S. J. Gee, B. D. Hammock

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We demonstrate the construction of an immunoarray to categorize, identify and quantitate different triazine herbicides or their environmental metabolites in water. In a first step cluster analysis is used to examine the performance of a small subset of antibodies out of a larger library, and hence to select a small array that is capable of categorizing the triazine herbicides into different groups. At the 1 ppb level it is possible to categorize an analyte as a chloro-s-triazine, a hydroxy metabolite, or as a methoxy/methylthio substituted triazine when using only 2-4 antibodies. At higher concentrations even the identity of the triazine can be determined with the same number of antibodies. A selected combination of antibodies is then used to identify and quantify unknowns by comparing their immunoassay responses to an array of calibration curves and using the maximum likelihood criterion.

Original languageEnglish (US)
Pages (from-to)291-303
Number of pages13
JournalAnalytica Chimica Acta
Volume319
Issue number3
DOIs
StatePublished - Feb 9 1996

Fingerprint

Triazines
triazine
antibody
Antibodies
Herbicides
Metabolites
herbicide
metabolite
immunoassay
Cluster analysis
Immunoassay
Calibration
Maximum likelihood
Libraries
Cluster Analysis
cluster analysis
calibration
Water
water

Keywords

  • Environmental analysis
  • Herbicides
  • Immunoarray construction
  • Triazine herbicides

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes. / Wortberg, M.; Jones, G.; Kreissig, S. B.; Rocke, David M; Gee, S. J.; Hammock, B. D.

In: Analytica Chimica Acta, Vol. 319, No. 3, 09.02.1996, p. 291-303.

Research output: Contribution to journalArticle

Wortberg, M. ; Jones, G. ; Kreissig, S. B. ; Rocke, David M ; Gee, S. J. ; Hammock, B. D. / An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes. In: Analytica Chimica Acta. 1996 ; Vol. 319, No. 3. pp. 291-303.
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