An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria

Zulfiqar A. Malik; Sumaira Amir

doi:10.1007/s12010-011-9394-9

An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria

Zulfiqar A. Malik, Sumaira Amir

Biochemistry and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.

Original language	English (US)
Pages (from-to)	1779-1788
Number of pages	10
Journal	Applied Biochemistry and Biotechnology
Volume	165
Issue number	7-8
DOIs	https://doi.org/10.1007/s12010-011-9394-9
State	Published - Dec 1 2011

Keywords

Apolipophorin III
Hemolymph
Locusta migratoria
Schistocerca gregaria

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

Access to Document

10.1007/s12010-011-9394-9

Cite this

@article{aff8b49c494c47beb690cd0339585b7c,

title = "An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria",

abstract = "Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.",

keywords = "Apolipophorin III, Hemolymph, Locusta migratoria, Schistocerca gregaria",

author = "Malik, {Zulfiqar A.} and Sumaira Amir",

year = "2011",

month = dec,

day = "1",

doi = "10.1007/s12010-011-9394-9",

language = "English (US)",

volume = "165",

pages = "1779--1788",

journal = "Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology",

issn = "0273-2289",

publisher = "Humana Press",

number = "7-8",

}

TY - JOUR

T1 - An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria

AU - Malik, Zulfiqar A.

AU - Amir, Sumaira

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.

AB - Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.

KW - Apolipophorin III

KW - Hemolymph

KW - Locusta migratoria

KW - Schistocerca gregaria

UR - http://www.scopus.com/inward/record.url?scp=81955167908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81955167908&partnerID=8YFLogxK

U2 - 10.1007/s12010-011-9394-9

DO - 10.1007/s12010-011-9394-9

M3 - Article

C2 - 21976149

AN - SCOPUS:81955167908

VL - 165

SP - 1779

EP - 1788

JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

SN - 0273-2289

IS - 7-8

ER -

An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this