Abstract
Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1779-1788 |
| Number of pages | 10 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 165 |
| Issue number | 7-8 |
| DOIs | |
| State | Published - Dec 1 2011 |
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Keywords
- Apolipophorin III
- Hemolymph
- Locusta migratoria
- Schistocerca gregaria
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
Cite this
An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria. / Malik, Zulfiqar A.; Amir, Sumaira.
In: Applied Biochemistry and Biotechnology, Vol. 165, No. 7-8, 01.12.2011, p. 1779-1788.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An apolipophorin III protein from the hemolymph of desert locust, Schistocerca gregaria
AU - Malik, Zulfiqar A.
AU - Amir, Sumaira
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.
AB - Apolipophorin III (apoLp-III) from insects and apolipoprotein A-I from humans, are major component of the lipoprotein and share various properties. ApoLp-III is an abundant hemolymph protein. Besides its crucial role in lipid transport, apoLp-III is able to associate with fungal and bacterial membranes and stimulate cellular immune responses. ApoLp-III was isolated and purified from the hemolymph of desert locust Schistocerca gregaria by ion-exchange and reversed-phase chromatography. The purity and the molecular weight of apoLp-III were determined at ∼19,000 Da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. According to similarities in the amino terminal sequence, molar mass and retention on reversed-phase analytical HPLC column, this protein is a Schistocerca gregaria homologue of Locusta migratoria apoLp-III.
KW - Apolipophorin III
KW - Hemolymph
KW - Locusta migratoria
KW - Schistocerca gregaria
UR - http://www.scopus.com/inward/record.url?scp=81955167908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=81955167908&partnerID=8YFLogxK
U2 - 10.1007/s12010-011-9394-9
DO - 10.1007/s12010-011-9394-9
M3 - Article
C2 - 21976149
AN - SCOPUS:81955167908
VL - 165
SP - 1779
EP - 1788
JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
SN - 0273-2289
IS - 7-8
ER -