An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the 'Wobble' base in the anticodon of tRNAs

W. D. Heyer, P. Thuriaux, J. Kohli

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The screening of antisuppressor mutants of the yeast Schizosaccharomyces pombe has been successfully accomplished with high resolution liquid chromatographic methods for the analysis of tRNA nucleosides. Antisuppressor mutations reduce or abolish the function of nonsense suppressor-tRNAs of other informational suppressors. Nonradioactive or 35S-labeled unfractionated tRNA from various strains was digested to nucleosides and analyzed by high performance liquid chromatography. The mutant sin3 has lost the nucleoside 5-(methoxycarbonylmethyl)-2-thiouridine from its tRNA in comparison to parental strains. In eukaryotes this nucleoside is found at the first position of the anticodon (wobble position) in several isoacceptor tRNAs that preferentially recognize codons ending with adenosine. The sin3 mutation reduces the efficiency of UGA and UAA suppressor tRNA(Ser) and suppressor tRNA(Leu). The genetic cosegregation of modification loss, antisuppressor phenotype, and a change in cell size is demonstrated. This indicates that a single mutation in the structural gene for a tRNA modification enzyme causes the three different phenotypes.

Original languageEnglish (US)
Pages (from-to)2856-2862
Number of pages7
JournalJournal of Biological Chemistry
Volume259
Issue number5
StatePublished - 1984
Externally publishedYes

Fingerprint

Anticodon
Schizosaccharomyces
Transfer RNA
Mutation
Nucleosides
RNA, Transfer, Leu
Phenotype
High performance liquid chromatography
Eukaryota
Cell Size
Codon
Liquid Chromatography
Yeast
Adenosine
Screening
Genes
Yeasts
High Pressure Liquid Chromatography
Liquids
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the 'Wobble' base in the anticodon of tRNAs. / Heyer, W. D.; Thuriaux, P.; Kohli, J.

In: Journal of Biological Chemistry, Vol. 259, No. 5, 1984, p. 2856-2862.

Research output: Contribution to journalArticle

@article{31349c3614a94461a00dd5200ab22fab,
title = "An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the 'Wobble' base in the anticodon of tRNAs",
abstract = "The screening of antisuppressor mutants of the yeast Schizosaccharomyces pombe has been successfully accomplished with high resolution liquid chromatographic methods for the analysis of tRNA nucleosides. Antisuppressor mutations reduce or abolish the function of nonsense suppressor-tRNAs of other informational suppressors. Nonradioactive or 35S-labeled unfractionated tRNA from various strains was digested to nucleosides and analyzed by high performance liquid chromatography. The mutant sin3 has lost the nucleoside 5-(methoxycarbonylmethyl)-2-thiouridine from its tRNA in comparison to parental strains. In eukaryotes this nucleoside is found at the first position of the anticodon (wobble position) in several isoacceptor tRNAs that preferentially recognize codons ending with adenosine. The sin3 mutation reduces the efficiency of UGA and UAA suppressor tRNA(Ser) and suppressor tRNA(Leu). The genetic cosegregation of modification loss, antisuppressor phenotype, and a change in cell size is demonstrated. This indicates that a single mutation in the structural gene for a tRNA modification enzyme causes the three different phenotypes.",
author = "Heyer, {W. D.} and P. Thuriaux and J. Kohli",
year = "1984",
language = "English (US)",
volume = "259",
pages = "2856--2862",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "5",

}

TY - JOUR

T1 - An antisuppressor mutation of Schizosaccharomyces pombe affects the post-transcriptional modification of the 'Wobble' base in the anticodon of tRNAs

AU - Heyer, W. D.

AU - Thuriaux, P.

AU - Kohli, J.

PY - 1984

Y1 - 1984

N2 - The screening of antisuppressor mutants of the yeast Schizosaccharomyces pombe has been successfully accomplished with high resolution liquid chromatographic methods for the analysis of tRNA nucleosides. Antisuppressor mutations reduce or abolish the function of nonsense suppressor-tRNAs of other informational suppressors. Nonradioactive or 35S-labeled unfractionated tRNA from various strains was digested to nucleosides and analyzed by high performance liquid chromatography. The mutant sin3 has lost the nucleoside 5-(methoxycarbonylmethyl)-2-thiouridine from its tRNA in comparison to parental strains. In eukaryotes this nucleoside is found at the first position of the anticodon (wobble position) in several isoacceptor tRNAs that preferentially recognize codons ending with adenosine. The sin3 mutation reduces the efficiency of UGA and UAA suppressor tRNA(Ser) and suppressor tRNA(Leu). The genetic cosegregation of modification loss, antisuppressor phenotype, and a change in cell size is demonstrated. This indicates that a single mutation in the structural gene for a tRNA modification enzyme causes the three different phenotypes.

AB - The screening of antisuppressor mutants of the yeast Schizosaccharomyces pombe has been successfully accomplished with high resolution liquid chromatographic methods for the analysis of tRNA nucleosides. Antisuppressor mutations reduce or abolish the function of nonsense suppressor-tRNAs of other informational suppressors. Nonradioactive or 35S-labeled unfractionated tRNA from various strains was digested to nucleosides and analyzed by high performance liquid chromatography. The mutant sin3 has lost the nucleoside 5-(methoxycarbonylmethyl)-2-thiouridine from its tRNA in comparison to parental strains. In eukaryotes this nucleoside is found at the first position of the anticodon (wobble position) in several isoacceptor tRNAs that preferentially recognize codons ending with adenosine. The sin3 mutation reduces the efficiency of UGA and UAA suppressor tRNA(Ser) and suppressor tRNA(Leu). The genetic cosegregation of modification loss, antisuppressor phenotype, and a change in cell size is demonstrated. This indicates that a single mutation in the structural gene for a tRNA modification enzyme causes the three different phenotypes.

UR - http://www.scopus.com/inward/record.url?scp=0021186152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021186152&partnerID=8YFLogxK

M3 - Article

VL - 259

SP - 2856

EP - 2862

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 5

ER -