An animal model for Lyme arthritis

Stephen W Barthold, K. D. Moody, G. A. Terwilliger, R. O. Jacoby, A. C. Steere

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A model of Lyme arthritis has been developed in laboratory rats. Intraperitoneal inoculation of a low-passage tick isolate of B. burgdorferi into neonatal and weanling LEW/N rats resulted in multisystemic infection and arthritis. Spirochetes were isolated from blood, liver, kidney, spleen, brain, and joints of inoculated rats. Arthritis, associated with the presence of spirochetes, developed in multiple joints by day 14 and persisted through day 90 after inoculation. Arthritic lesions resembled those found in human Lyme disease lesions. Lesions were not found in other organs, although spirochetes were present. Neonatal F344 and SD rats were also susceptible to infection and induction of arthritis. Three different isolates of B. burgdorferi were shown to be pathogenic. Pathogenicity of one isolate was retained after at least 11 in vitro passages. Formalin-killed spirochetes were not pathogenic. Other features of the Lyme disease complex have yet to be seen in the rat, but long-term studies are required to completely define the rat model. This highly reproducible model should allow in-depth studies on the pathogenetic mechanisms of this important human disease.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume539
StatePublished - 1988
Externally publishedYes

Fingerprint

Lyme Disease
Spirochaetales
Rats
Animals
Animal Models
Arthritis
Joints
Inbred F344 Rats
Ticks
Infection
Formaldehyde
Virulence
Liver
Spleen
Animal Model
Rat
Brain
Blood
Kidney
Lesion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Barthold, S. W., Moody, K. D., Terwilliger, G. A., Jacoby, R. O., & Steere, A. C. (1988). An animal model for Lyme arthritis. Annals of the New York Academy of Sciences, 539, 265-273.

An animal model for Lyme arthritis. / Barthold, Stephen W; Moody, K. D.; Terwilliger, G. A.; Jacoby, R. O.; Steere, A. C.

In: Annals of the New York Academy of Sciences, Vol. 539, 1988, p. 265-273.

Research output: Contribution to journalArticle

Barthold, SW, Moody, KD, Terwilliger, GA, Jacoby, RO & Steere, AC 1988, 'An animal model for Lyme arthritis', Annals of the New York Academy of Sciences, vol. 539, pp. 265-273.
Barthold SW, Moody KD, Terwilliger GA, Jacoby RO, Steere AC. An animal model for Lyme arthritis. Annals of the New York Academy of Sciences. 1988;539:265-273.
Barthold, Stephen W ; Moody, K. D. ; Terwilliger, G. A. ; Jacoby, R. O. ; Steere, A. C. / An animal model for Lyme arthritis. In: Annals of the New York Academy of Sciences. 1988 ; Vol. 539. pp. 265-273.
@article{bb173c15301f43aea41d23c3fdce1d72,
title = "An animal model for Lyme arthritis",
abstract = "A model of Lyme arthritis has been developed in laboratory rats. Intraperitoneal inoculation of a low-passage tick isolate of B. burgdorferi into neonatal and weanling LEW/N rats resulted in multisystemic infection and arthritis. Spirochetes were isolated from blood, liver, kidney, spleen, brain, and joints of inoculated rats. Arthritis, associated with the presence of spirochetes, developed in multiple joints by day 14 and persisted through day 90 after inoculation. Arthritic lesions resembled those found in human Lyme disease lesions. Lesions were not found in other organs, although spirochetes were present. Neonatal F344 and SD rats were also susceptible to infection and induction of arthritis. Three different isolates of B. burgdorferi were shown to be pathogenic. Pathogenicity of one isolate was retained after at least 11 in vitro passages. Formalin-killed spirochetes were not pathogenic. Other features of the Lyme disease complex have yet to be seen in the rat, but long-term studies are required to completely define the rat model. This highly reproducible model should allow in-depth studies on the pathogenetic mechanisms of this important human disease.",
author = "Barthold, {Stephen W} and Moody, {K. D.} and Terwilliger, {G. A.} and Jacoby, {R. O.} and Steere, {A. C.}",
year = "1988",
language = "English (US)",
volume = "539",
pages = "265--273",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - An animal model for Lyme arthritis

AU - Barthold, Stephen W

AU - Moody, K. D.

AU - Terwilliger, G. A.

AU - Jacoby, R. O.

AU - Steere, A. C.

PY - 1988

Y1 - 1988

N2 - A model of Lyme arthritis has been developed in laboratory rats. Intraperitoneal inoculation of a low-passage tick isolate of B. burgdorferi into neonatal and weanling LEW/N rats resulted in multisystemic infection and arthritis. Spirochetes were isolated from blood, liver, kidney, spleen, brain, and joints of inoculated rats. Arthritis, associated with the presence of spirochetes, developed in multiple joints by day 14 and persisted through day 90 after inoculation. Arthritic lesions resembled those found in human Lyme disease lesions. Lesions were not found in other organs, although spirochetes were present. Neonatal F344 and SD rats were also susceptible to infection and induction of arthritis. Three different isolates of B. burgdorferi were shown to be pathogenic. Pathogenicity of one isolate was retained after at least 11 in vitro passages. Formalin-killed spirochetes were not pathogenic. Other features of the Lyme disease complex have yet to be seen in the rat, but long-term studies are required to completely define the rat model. This highly reproducible model should allow in-depth studies on the pathogenetic mechanisms of this important human disease.

AB - A model of Lyme arthritis has been developed in laboratory rats. Intraperitoneal inoculation of a low-passage tick isolate of B. burgdorferi into neonatal and weanling LEW/N rats resulted in multisystemic infection and arthritis. Spirochetes were isolated from blood, liver, kidney, spleen, brain, and joints of inoculated rats. Arthritis, associated with the presence of spirochetes, developed in multiple joints by day 14 and persisted through day 90 after inoculation. Arthritic lesions resembled those found in human Lyme disease lesions. Lesions were not found in other organs, although spirochetes were present. Neonatal F344 and SD rats were also susceptible to infection and induction of arthritis. Three different isolates of B. burgdorferi were shown to be pathogenic. Pathogenicity of one isolate was retained after at least 11 in vitro passages. Formalin-killed spirochetes were not pathogenic. Other features of the Lyme disease complex have yet to be seen in the rat, but long-term studies are required to completely define the rat model. This highly reproducible model should allow in-depth studies on the pathogenetic mechanisms of this important human disease.

UR - http://www.scopus.com/inward/record.url?scp=0023721158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023721158&partnerID=8YFLogxK

M3 - Article

C2 - 3263827

AN - SCOPUS:0023721158

VL - 539

SP - 265

EP - 273

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -