An air pressure system for the injection of tracer substances into the brain

David G Amaral, J. L. Price

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

A system for injecting tracers and other substances into the brain through glass micropipettes using mechanically or electromechanically regulated air pressure as the driving force is described. The system has a number of advantages over currently used devices including: accurate ejection of a wide range of volumes (2 nl to several microliters); the capability to inject all currently utilized tracers; and, the direct visualization of the volume of solution injected. This system has proven reliable for a number of tract tracing experiments ranging from the injection of tracers into deep structures of the monkey brain to injecting small quantities of tracers into the brains of fetal rats.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalJournal of Neuroscience Methods
Volume9
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • axonal tracers
  • electromechanical valving system
  • micropipettes
  • pressure injection system
  • tracer injection

ASJC Scopus subject areas

  • Neuroscience(all)

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