Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis

Shimon Harrus, Trevor Waner, Itzhak Aizenberg, Janet E Foley, Amy M. Poland, Hylton Bark

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with E. canis. At least one of the three samples (spleen, bone marrow, and blood) from four of the six dogs was PCR positive. The spleens of all four of these dogs were PCR positive, and the bone marrow and blood of two of the four dogs were PCR positive. Indirect immunofluorescent-antibody titers increased progressively during the first 5 months postinfection, remained high for an additional period of more than 11 months, and declined thereafter, suggesting that the dogs were recovering from the disease. Five of the dogs remained seropositive 34 months postinfection. The data obtained in this study demonstrate for the first time that clinically healthy dogs in the subclinical phase of CME are carriers of the rickettsia. It was shown that dogs can harbor E. canis for years without developing the chronic clinical disease and that dogs can eliminate the parasite and recover from CME without medical treatment. Our findings suggest that the spleen is the organ most likely to harbor E. canis parasites during the subclinical phase and the last organ to accommodate the parasite before elimination. It was concluded that PCR of DNA extracted from splenic aspirates is a reliable method for determining the carrier state of CME.

Original languageEnglish (US)
Pages (from-to)73-76
Number of pages4
JournalJournal of Clinical Microbiology
Volume36
Issue number1
StatePublished - Jan 1998

Fingerprint

Ehrlichia canis
Dogs
Ehrlichiosis
DNA
Infection
Canidae
Polymerase Chain Reaction
Parasites
Spleen
Bone Marrow
Carrier State
Rickettsia
Antibodies
Chronic Disease

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Harrus, S., Waner, T., Aizenberg, I., Foley, J. E., Poland, A. M., & Bark, H. (1998). Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. Journal of Clinical Microbiology, 36(1), 73-76.

Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. / Harrus, Shimon; Waner, Trevor; Aizenberg, Itzhak; Foley, Janet E; Poland, Amy M.; Bark, Hylton.

In: Journal of Clinical Microbiology, Vol. 36, No. 1, 01.1998, p. 73-76.

Research output: Contribution to journalArticle

Harrus, S, Waner, T, Aizenberg, I, Foley, JE, Poland, AM & Bark, H 1998, 'Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis', Journal of Clinical Microbiology, vol. 36, no. 1, pp. 73-76.
Harrus, Shimon ; Waner, Trevor ; Aizenberg, Itzhak ; Foley, Janet E ; Poland, Amy M. ; Bark, Hylton. / Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. In: Journal of Clinical Microbiology. 1998 ; Vol. 36, No. 1. pp. 73-76.
@article{24933c4748564d55bd5f0586195d5e5d,
title = "Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis",
abstract = "In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with E. canis. At least one of the three samples (spleen, bone marrow, and blood) from four of the six dogs was PCR positive. The spleens of all four of these dogs were PCR positive, and the bone marrow and blood of two of the four dogs were PCR positive. Indirect immunofluorescent-antibody titers increased progressively during the first 5 months postinfection, remained high for an additional period of more than 11 months, and declined thereafter, suggesting that the dogs were recovering from the disease. Five of the dogs remained seropositive 34 months postinfection. The data obtained in this study demonstrate for the first time that clinically healthy dogs in the subclinical phase of CME are carriers of the rickettsia. It was shown that dogs can harbor E. canis for years without developing the chronic clinical disease and that dogs can eliminate the parasite and recover from CME without medical treatment. Our findings suggest that the spleen is the organ most likely to harbor E. canis parasites during the subclinical phase and the last organ to accommodate the parasite before elimination. It was concluded that PCR of DNA extracted from splenic aspirates is a reliable method for determining the carrier state of CME.",
author = "Shimon Harrus and Trevor Waner and Itzhak Aizenberg and Foley, {Janet E} and Poland, {Amy M.} and Hylton Bark",
year = "1998",
month = "1",
language = "English (US)",
volume = "36",
pages = "73--76",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis

AU - Harrus, Shimon

AU - Waner, Trevor

AU - Aizenberg, Itzhak

AU - Foley, Janet E

AU - Poland, Amy M.

AU - Bark, Hylton

PY - 1998/1

Y1 - 1998/1

N2 - In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with E. canis. At least one of the three samples (spleen, bone marrow, and blood) from four of the six dogs was PCR positive. The spleens of all four of these dogs were PCR positive, and the bone marrow and blood of two of the four dogs were PCR positive. Indirect immunofluorescent-antibody titers increased progressively during the first 5 months postinfection, remained high for an additional period of more than 11 months, and declined thereafter, suggesting that the dogs were recovering from the disease. Five of the dogs remained seropositive 34 months postinfection. The data obtained in this study demonstrate for the first time that clinically healthy dogs in the subclinical phase of CME are carriers of the rickettsia. It was shown that dogs can harbor E. canis for years without developing the chronic clinical disease and that dogs can eliminate the parasite and recover from CME without medical treatment. Our findings suggest that the spleen is the organ most likely to harbor E. canis parasites during the subclinical phase and the last organ to accommodate the parasite before elimination. It was concluded that PCR of DNA extracted from splenic aspirates is a reliable method for determining the carrier state of CME.

AB - In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with E. canis. At least one of the three samples (spleen, bone marrow, and blood) from four of the six dogs was PCR positive. The spleens of all four of these dogs were PCR positive, and the bone marrow and blood of two of the four dogs were PCR positive. Indirect immunofluorescent-antibody titers increased progressively during the first 5 months postinfection, remained high for an additional period of more than 11 months, and declined thereafter, suggesting that the dogs were recovering from the disease. Five of the dogs remained seropositive 34 months postinfection. The data obtained in this study demonstrate for the first time that clinically healthy dogs in the subclinical phase of CME are carriers of the rickettsia. It was shown that dogs can harbor E. canis for years without developing the chronic clinical disease and that dogs can eliminate the parasite and recover from CME without medical treatment. Our findings suggest that the spleen is the organ most likely to harbor E. canis parasites during the subclinical phase and the last organ to accommodate the parasite before elimination. It was concluded that PCR of DNA extracted from splenic aspirates is a reliable method for determining the carrier state of CME.

UR - http://www.scopus.com/inward/record.url?scp=0031984815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031984815&partnerID=8YFLogxK

M3 - Article

C2 - 9431923

AN - SCOPUS:0031984815

VL - 36

SP - 73

EP - 76

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -