Amplification of Coccidioidal DNA in Clinical Specimens by PCR

Suzanne M. Johnson, Keira A. Simmons, Demosthenes Pappagianis

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.

Original languageEnglish (US)
Pages (from-to)1982-1985
Number of pages4
JournalJournal of Clinical Microbiology
Issue number5
StatePublished - May 2004

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology


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