Amplification-mutagenesis: Evidence that "directed" adaptive mutation and general hypermutability result from growth with a selected gene amplification

Heather Hendrickson, E. Susan Slechta, Ulfar Bergthorsson, Dan I. Andersson, John R. Roth

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

When a particular lac mutant of Escherichia coli starves in the presence of lactose, nongrowing cells appear to direct mutations preferentially to sites that allow growth (adaptive mutation). This observation suggested that growth limitation stimulates mutability. Evidence is provided here that this behavior is actually caused by a standard Darwinian process in which natural selection acts in three sequential steps. First, growth limitation favors growth of a subpopulation with an amplification of the mutant lac gene; next, it favors cells with a lac+ revertant allele within the amplified array. Finally, it favors loss of mutant copies until a stable haploid lac+ revertant arises and overgrows the colony. By increasing the lac copy number, selection enhances the likelihood of reversion within each developing clone. This sequence of events appears to direct mutations to useful sites. General mutagenesis is a side-effect of growth with an amplification (SOS induction). The F′ plasmid, which carries lac, contributes by stimulating gene duplication and amplification. Selective stress has no direct effect on mutation rate or target specificity, but acts to favor a succession of cell types with progressively improved growth on lactose. The sequence of events - amplification, mutation, segregation - may help to explain both the origins of some cancers and the evolution of new genes under selection.

Original languageEnglish (US)
Pages (from-to)2164-2169
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number4
DOIs
StatePublished - Feb 19 2002
Externally publishedYes

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Gene Amplification
Mutagenesis
Mutation
Growth
Lactose
F Factor
Lac Operon
Gene Duplication
Genetic Selection
Haploidy
Mutation Rate
Clone Cells
Alleles
Escherichia coli
Genes
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Amplification-mutagenesis : Evidence that "directed" adaptive mutation and general hypermutability result from growth with a selected gene amplification. / Hendrickson, Heather; Slechta, E. Susan; Bergthorsson, Ulfar; Andersson, Dan I.; Roth, John R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 4, 19.02.2002, p. 2164-2169.

Research output: Contribution to journalArticle

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